Jing Yang

Publications

1 Accurate HLA Typing at High-Digit Resolution from NGS Data

Authors: Yan Zhang, Yazhi Huang, Jing Yang, Dingge Ying, Vorasuk Shotelersuk, Nattiya Hirankarn, Pak Chung Sham, Yu Lung Lau, Wanling Yang

Abstract:

Human leukocyte antigen (HLA) typing from next generation sequencing (NGS) data has the potential for applications in clinical laboratories and population genetic studies. Here we introduce a novel technique for HLA typing from NGS data based on read-mapping using a comprehensive reference panel containing all known HLA alleles and de novo assembly of the gene-specific short reads. An accurate HLA typing at high-digit resolution was achieved when it was tested on publicly available NGS data, outperforming other newly-developed tools such as HLAminer and PHLAT.

Keywords: whole exome sequencing, human leukocyte antigens, HLA typing, next generation sequencing

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Abstracts

1 Accurate HLA Typing at High-Digit Resolution from NGS Data

Authors: Yan Zhang, Yazhi Huang, Jing Yang, Dingge Ying, Vorasuk Shotelersuk, Nattiya Hirankarn, Pak Chung Sham, Yu Lung Lau, Wanling Yang

Abstract:

Human leukocyte antigen (HLA) typing from next generation sequencing (NGS) data has the potential for applications in clinical laboratories and population genetic studies. Here we introduce a novel technique for HLA typing from NGS data based on read-mapping using a comprehensive reference panel containing all known HLA alleles and de novo assembly of the gene-specific short reads. An accurate HLA typing at high-digit resolution was achieved when it was tested on publicly available NGS data, outperforming other newly-developed tools such as HLAminer and PHLAT.

Keywords: Next Generation Sequencing, whole exome sequencing, human leukocyte antigens, HLA typing

Procedia PDF Downloads 351