Commenced in January 2007
Frequency: Monthly
Edition: International
Paper Count: 2

Antinociceptive Related Publications

2 Effect of Geum Kokanicum Total Extract on Induced Nociception and Inflammation in Male Mice

Authors: M. Ramezani, S. Ghaderifard, HR. Monsef-Esfahani, S. Nasri

Abstract:

The aim of this study is evaluating the antinociceptive and anti-inflamatory activity of Geum kokanicum. After determination total extract LD50, different doses of extract were chosen for intrapritoneal injections. In inflammation test, male NMRI mice were divided into 6 groups: control (normal saline), positive control (Dexamethasone 15mg/kg), and total extract (0.025, 0.05, 0.1, and 0.2 gr/kg). The inflammation was produced by xyleneinduced edema. In order to evaluate the antinociceptive effect of total extract, formalin test was used. Mice were divided into 6 groups: control, positive control (morphine 10mg/kg), and 4 groups which received total extract. Then they received Formalin. The animals were observed for the reaction to pain. Data were analyzed using One-way ANOVA followed by Tukey-Kramer multiple comparison test. LD50 was 1 gr/kg. Data indicated that 0.5,0.1 and 0.2 gr/kg doses of total extract have particular antinociceptive and antiinflammatory effects in a comparison with control (P<0.001). The most effective dose was 0.2 gr/kg which did not show any significant difference in a comparison with positive control. Results indicated that total extract can inhibit nociception in the first and second phase. The antinociceptive effects in high doses are the same as morphine as a strong analgesic substance. TLC chromatography indicated presence of steroids and triterpenoids in this plant. The effects of extract may be related to presence of these compounds.

Keywords: mice, Anti-Inflammatory, Antinociceptive, Geum kokanicum

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1 Antinociceptive and Anti-inflammatory Effects of Hydroalcohol Extract of Vitex agnus castus Fruit

Authors: M. Ramezani, Gh. Amin, E. Jalili

Abstract:

In present study the effects of anti-inflammatory and antinociceptive of vitex hydro-alcoholic extract were evaluated on male mice. In inflammatory test mice were divided into 7 groups: first group was control. The second group, positive control group, received dexamethasone (15 mg/kg) and the other five groups received different doses of hydroalcohol extract of Vitex fruit (265, 365, 465, 565, and 665 mg/kg). The inflammation was caused by xylene-induced ear edema. Formalin test was used for evaluation of antinociceptive effect of extract. In this test, mice were divided into 7 groups: control, morphine (10mg/kg) as positive control group, and Vitex extract groups ((265, 365, 465, 565, and 665 mg/kg). All drugs were administered intrapritoneally, 30 min before each test. The data were analyzed using one-way ANOVA followed by Tukey-kramer multiple comparison test. Results have shown significant antiinflammatory effects of extract at all dosed as compared with control (P<0.05). In the first phase of formalin test (0-5 min) none doses of extract could inhibit acute pain, but in the second phase (15-30 min) chronic pain decreased at 265, 365, 465, and 565 mg/kg doses (P<0.05). The results of this research indicated that Vitex extract remarkably inhibited inflammation and second phase of nociception (inflammatory pain) and can be used for treatment of inflammatory diseases.

Keywords: mice, Anti-Inflammatory, Antinociceptive, Vitexagnus castus

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