Commenced in January 2007
Frequency: Monthly
Edition: International
Paper Count: 2

SHEDs Related Abstracts

2 Role of Interleukin 6 on Cell Differentiations in Stem Cells Isolated from Human Exfoliated Deciduous Teeth

Authors: Nunthawan Nowwarote, Waleerat Sukarawan, Prasit Pavasant, Thanaphum Osathanon

Abstract:

Interleukin 6 (IL-6) is a multifunctional cytokine, regulating various biological responses in several tissues. A Recent study shows that IL-6 plays a role in stemness maintenance in stem cells isolated from human exfoliated deciduous teeth (SHEDs). However, the role of IL-6 on cell differentiation in SHEDs remains unknown. The present study investigated the effect of IL-6 on SHEDs differentiation. Cells were isolated from dental pulp tissues of human deciduous teeth. Flow cytometry was used to determined mesenchymal stem cell marker expression, and the multipotential differentiation (osteogenic, adipogenic and neurogenic lineage ) was also determined. The mRNA was determined using real-time quantitative polymerase chain reaction, and the phenotypes were confirmed by chemical and immunofluorescence staining. Results demonstrated that SHEDs expressed CD44, CD73, CD90, CD105 but not CD45. Further, the up-regulation of osteogenic, adipogenic and neurogenic marker genes was observed upon maintaining cells in osteogenic, adipogenic and neurogenic induction medium, respectively. The addition of IL-6 induced osteogenic by up-regulated osteogenic marker gene also increased in vitro mineralization. Under neurogenic medium supplement with IL-6, up-regulated neurogenic marker. Whereas, an addition of IL-6 attenuated adipogenic differentiation by SHEDs. In conclusion, this evidence implies that IL-6 may participate in cells differentiation ability of SHEDs.

Keywords: IL-6, dental pulp, SHEDs, cell differentiations

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1 The Importance of Zenithal Lighting Systems for Natural Light Gains and for Local Energy Generation in Brazil

Authors: Ana Paula Esteves, Diego S. Caetano, Louise L. B. Lomardo

Abstract:

This paper presents an approach on the advantages of using adequate coverage in the zenithal lighting typology in various areas of architectural production, while at the same time to encourage to the design concerns inherent in this choice of roofing in Brazil. Understanding that sustainability needs to cover several aspects, a roofing system such as zenithal lighting system can contribute to the provision of better quality natural light for the interior of the building, which is related to the good health and welfare; it will also be able to contribute for the sustainable aspects and environmental needs, when it allows the generation of energy in semitransparent or opacity photovoltaic solutions and economize the artificial lightning. When the energy balance in the building is positive, that is, when the building generates more energy than it consumes, it may fit into the Net Zero Energy Building concept. The zenithal lighting systems could be an important ally in Brazil, when solved the burden of heat gains, participate in the set of pro-efficiency actions in search of "zero energy buildings". The paper presents comparative three cases of buildings that have used this feature in search of better environmental performance, both in light comfort and sustainability as a whole. Two of these buildings are examples in Europe: the Notley Green School in the UK and the Isofóton factory in Spain. The third building with these principles of shed´s roof is located in Brazil: the Ipel´s factory in São Paulo.

Keywords: Natural Lighting, SHEDs, net zero energy building, semi-transparent photovoltaics

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