WASET
	%0 Journal Article
	%A P. I. Bobyleva and  E. R. Andreeva and  I. V. Andrianova and  E. V. Maslova and  L.B. Buravkova
	%D 2014
	%J International Journal of Bioengineering and Life Sciences
	%B World Academy of Science, Engineering and Technology
	%I Open Science Index 93, 2014
	%T Stroma-Providing Activity of Adipose Derived Mesenchymal Stromal Cells in Tissue-Related O2 Microenvironment
	%U https://publications.waset.org/pdf/9999276
	%V 93
	%X This work studied the ability of adipose tissue-derived
mesenchymal stromal cells (MSCs) to form stroma for expansion of
cord blood hematopoietic cells. We showed that 72-hour interaction
of MSCs with cord blood mononuclear cells (MNCs) in vitro at
atmospheric (20%) and low (5%) O2 conditions increased the
expression of ICAM-1, HCAM (at the beginning of interaction) on
MSCs. Viability of MSCs and MNCs were maintained at high level.
Adhesion of MNCs to MSCs was faster at 20% O2. MSCs promoted
the proliferation of adhered MNCs to form the suspension containing
great number of hematopoietic colony-forming units, and this effect
was more pronounced at 5% O2. Thus, adipose-derived MSCs
supplied sufficient stromal support to cord blood MNCs both at 20%
and 5% О2, providing their adhesion with further expansion of new
generation of different hematopoietic lineages.

	%P 544 - 548