Vandana Bali and Manab B. Bera and Parmjit S. Panesar
Utilization of AgroIndustrial Byproducts for Bacteriocin Production Using Newly Isolated Enterococcus faecium BS13
562 - 566
2014
8
6
International Journal of Agricultural and Biosystems Engineering
https://publications.waset.org/pdf/9998371
https://publications.waset.org/vol/90
World Academy of Science, Engineering and Technology
Microbial production of antimicrobials as biopreservatives is the major area of focus nowadays due to increased interest of consumers towards natural and safe preservation of ready to eat food products. The agroindustrial byproduct based medium and optimized process conditions can contribute in economical production of bacteriocins. Keeping this in view, the present investigation was carried out on agroindustrial byproducts utilization for the production of bacteriocin using Enterococcus faecium BS13 isolated from local fermented food. Different agroindustrial byproduct based carbon sources (whey, potato starch liquor, kinnow peel, deoiledrice bran and molasses), nitrogen sources (soya okra, pea pod and corn steep liquor), metal ions and surfactants were tested for optimal bacteriocin production. The effect of various process parameters such as pH, temperature, inoculum level, agitation and time were also tested on bacteriocin production. The optimized medium containing whey, supplemented with 4corn steep liquor and polysorbate80 displayed maximum bacteriocin activity with 2 inoculum, at pH 6.5, temperature 40oC under shaking conditions (100 rpm).
Open Science Index 90, 2014