W. Wanmolee and W. Sornlake and N. Laosiripojana and V. Champreda
Development of Efficient Fungal BiomassDegrading Enzyme Mixtures for Saccharification of Local Lignocellulosic Feedstock
69 - 73
2014
8
2
International Journal of Energy and Power Engineering
https://publications.waset.org/pdf/9997349
https://publications.waset.org/vol/86
World Academy of Science, Engineering and Technology
Conversion of lignocellulosic biomass is the basis process for production of fuels, chemicals and materials in the sustainable biorefinery industry. Saccharification of lignocellulosic biomass is an essential step which produces sugars for further conversion to target valueadded products e.g. bioethanol, bioplastic, gvalerolactone (GVL), 5hydroxymethylfuroic acid (HMF), levulinic acid, etc. The goal of this work was to develop an efficient enzyme for conversion of biomass to reducing sugar based on crude fungal enzyme from Chaetomium globosum BCC5776 produced by submerged fermentation and evaluate its activity comparing to a commercial Acremonium cellulase. Five local biomasses in Thailand rice straw, sugarcane bagasse, corncobs, corn stovers, and palm empty fruit bunches were pretreated and hydrolyzed with varying enzyme loadings. Saccharification of the biomass led to different reducing sugar levels from 115 mgg to 720 mgg from different types of biomass using cellulase dosage of 9 FPUg. The reducing sugar will be further employed as sugar feedstock for production of ethanol or commodity chemicals. This work demonstrated the use of promising enzyme candidate for conversion of local lignocellulosic biomass in biorefinery industry.
Open Science Index 86, 2014