%0 Journal Article
	%A R.Sangeetha and  T.Karunanithi
	%D 2010
	%J International Journal of Chemical and Molecular Engineering
	%B World Academy of Science, Engineering and Technology
	%I Open Science Index 37, 2010
	%T Optimization of Growth of Rhodobacter Sphaeroides Using Mixed Volatile Fatty Acidsby Response Surface Methodology
	%U https://publications.waset.org/pdf/9946
	%V 37
	%X A combination of photosynthetic bacteria along with
anaerobic acidogenic bacteria is an ideal option for efficient
hydrogen production. In the present study, the optimum
concentration of substrates for the growth of Rhodobacter
sphaeroides was found by response surface methodology. The
optimum combination of three individual fatty acids was determined
by Box Behnken design. Increase of volatile fatty acid concentration
decreased the growth. Combination of sodium acetate and sodium
propionate was most significant for the growth of the organism. The
results showed that a maximum biomass concentration of 0.916 g/l
was obtained when the concentrations of acetate, propionate and
butyrate were 0.73g/l,0.99g/l and 0.799g/l, respectively. The growth
was studied under an optimum concentration of volatile fatty acids
and at a light intensity of 3000 lux, initial pH of 7 and a temperature
of 35°C.The maximum biomass concentration of 0.92g/l was
obtained which verified the practicability of this optimization.
	%P 123 - 126