Lolita Tomsone and Zanda Kruma and Ruta Galoburda
Comparison of Different Solvents and Extraction Methods for Isolation of Phenolic Compounds from Horseradish Roots (Armoracia rusticana)
236 - 241
2012
6
4
International Journal of Agricultural and Biosystems Engineering
https://publications.waset.org/pdf/8584
https://publications.waset.org/vol/64
World Academy of Science, Engineering and Technology
Horseradish (Armoracia rusticana) is a perennial herb belonging to the Brassicaceae family and contains biologically active substances. The aim of the current research was to determine best method for extraction of phenolic compounds from horseradish roots showing high antiradical activity. Three genotypes (No. 105; No. 106 and variety ‘Turku’) of horseradish roots were extracted with eight different solvents nhexane, ethyl acetate, diethyl ether, 2propanol, acetone, ethanol (95), ethanol water acetic acid (80201 vvv) and ethanol water (8020 by volume) using two extraction methods (conventional and Soxhlet). As the best solvents ethanol and ethanol water solutions can be chosen. Although in Soxhlet extracts TPC was higher, scavenging activity of DPPH radicals did not increase. It can be concluded that using Soxhlet extraction method more compounds that are not effective antioxidants.
Open Science Index 64, 2012