TY - JFULL AU - Lolita Tomsone and Zanda Kruma and Ruta Galoburda PY - 2012/5/ TI - Comparison of Different Solvents and Extraction Methods for Isolation of Phenolic Compounds from Horseradish Roots (Armoracia rusticana) T2 - International Journal of Agricultural and Biosystems Engineering SP - 235 EP - 241 VL - 6 SN - 1307-6892 UR - https://publications.waset.org/pdf/8584 PU - World Academy of Science, Engineering and Technology NX - Open Science Index 64, 2012 N2 - Horseradish (Armoracia rusticana) is a perennial herb belonging to the Brassicaceae family and contains biologically active substances. The aim of the current research was to determine best method for extraction of phenolic compounds from horseradish roots showing high antiradical activity. Three genotypes (No. 105; No. 106 and variety ‘Turku’) of horseradish roots were extracted with eight different solvents: n-hexane, ethyl acetate, diethyl ether, 2-propanol, acetone, ethanol (95%), ethanol / water / acetic acid (80/20/1 v/v/v) and ethanol / water (80/20 by volume) using two extraction methods (conventional and Soxhlet). As the best solvents ethanol and ethanol / water solutions can be chosen. Although in Soxhlet extracts TPC was higher, scavenging activity of DPPH˙ radicals did not increase. It can be concluded that using Soxhlet extraction method more compounds that are not effective antioxidants. ER -