Commenced in January 2007
Paper Count: 30174
Regulatory Effects of Carbon Sources on Tabtoxin Production (A β-lactam Phytotoxin of Pseudomonas syringae pv. tabaci)
Abstract:The effects of divers carbon substrates were investigated for the tabtoxin production of an isolated pathogenic Pseudomonas syringae pv. tabaci, the causal agent of wildfire of tobacco and are discussed in relation to the bacterium growth. The isolated organism was grown in batch culture on Woolley's medium (28°C, 200 rpm, during 5 days). The growth has been measured by the optical density (OD) at 620 nm and the tabtoxin production quantified by Escherichia coli (K-12) bioassay technique. The growth and the tabtoxin production were both influenced by the substrates (sugars, amino acids, organic acids) used, each, as a sole carbon source and as a supplement for the same amino acids. The most significant quantities of tabtoxin were obtained in presence of some amino acids used as sole carbon source and/or as supplement.
Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1071013Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 2710
 D. K. Willis, J. J. Rich, T. G. Kinscherf, and T. Kitten, "Genetic regulation in plant pathogenic pseudomonads," Genetic Engineering, vol. 176, no. 24, pp. 167-193, Dec. 1994.
 R. E. Mitchell, "Structure: Bacterial," in Toxins in plant disease, R. D. Durbin, Ed. New York: Academic, 1981, pp. 259-293.
 E. E. Clayton, "Toxin produced by Bacterium tabacum and its relation to host range," J. Agric. Res., vol. 48, no. 5, pp. 411-426, Mar. 1934.
 D. K. Willis, T. M. Barta, and T. G. Kinscherf, "Genetics of toxin production and resistance in phytopathogenic bacteria,"Experientia, vol. 47, pp. 765-770, 1991.
 J. Johnson, and H.F. Murwin, "Experiments on the control of wildfire tobacco," Wis. Agr. Exp. Sta. Res. Bull., vol. 62, pp. 1925. 35.
 R. E. Mitchell, "Implications of toxins in the ecology and evolution of plant pathogenic microorganisms: bacteria," Experientia, vol. 47, no. 8, pp. 791-803, Aug. 1991.
 C. Levi, and R. D. Durbin, "The isolation and proprieties tabtoxinhydrolizing aminopeptidase from the periplasm of Pseudomonas syringae pv. tabaci," Physiol. Mol. Plant Pathol., vol. 28, no. 3, pp. 345-352, May 1986.
 M. D. Thomas, P. J. Langston-Unkefer, T. F. Uchytil, and R. D. Durbin, "Inhibition of glutamine synthetase from pea by tabtoxinine- β-lactam," Plant Physiol., vol. 71, no. 4, pp. 912-915, Apr. 1983.
 T. J. Knight, R. D. Durbin, and P. J. Langston-Unkefer, "Selfprotection of Pseudomonas syringae pv. tabaci from its toxin, tabtoxinine-β-lactam," J. Bacteriol., vol. 196, no. 5, pp. 1954-1959, May 1987.
 C. J. Unkefer, R. E. London, R. D. Durbin, T. F. Uchytil, and P. J. Langston-Unkefer, "The biosynthesis of tabtoxinine-β-lactam," J. Biol.Chem., vol. 262, pp. 4994-4999, 1987.
 11T. G. Kinscherf, R. H. Colman, T. M. Barta, and D. K. Willis, "Cloning and expression of the tabtoxin biosynthesis region from Pseudomonas syringae," J. Bacteriol., vol. 173, pp. 4124-4132, 1991.
 T. M. Barta, T. G. Kinscherf, and D. K. Willis, "Regulation of tabtoxin production by the lemA gene in Pseudomonas syringae," J. Bacteriol., vol. 174, no. 9, pp. 3021-3029, May 1992.
 C. L. Bender, F. Alar├│n-Chaidez, and D. C. Gross, "Pseudomonas syringae phytotoxins: mode of action, regulation, and biosynthesis by peptide and polyketide synthetases," Microbiol. Mol. Biol. Reviews, vol. 63, no. 2, pp. 266-292, June 1999.
 J. F. Martin, and P. Liras, "Organization and expression of genes involved in the biosynthesis of antibiotics and other secondary metabolites," Annu. Rev. Microbiol., vol. 43, pp. 173-206, Oct. 1989.
 M. J. Gasson, "Indicator technique for antimetabolite toxin production by phytopathogenic species of Pseudomonas," Appl. Envi. Microbiol., vol. 39, no. 1, pp. 25-29, Jan. 1980.
 D. W. Woolley, R. B. Pringle, and A. C. Braun, "Isolation of the phytopathogenic toxin of Pseudomonas tabaci, an antagonist of methionine," J. Biol. Chem., vol. 197, pp. 409-417, Feb. 1952.
 D. A. Palmer, and C. L. Bender, "Effects of environmental and nutritional factors on production of polyketide phytotoxin coronatine by Pseudomonas syringae pv. glycinea," Appl. Envi. Microbiol., vol. 59, no. 5, pp. 1619-1626, May 1993.
 D. C. Gross, "Regulation of syringomycin synthesis in Pseudomonas syringae pv. Syringae and defined conditions for its production," J. Appl. bacteriol., vol. 58, pp. 167-174, 1985.
 B. M├╝ller, A. H├ñdener, and C. Tamm, "Studies on the biosynthesis of tabtoxin (wildfire toxin). Origin of the carbonyl C-atom of the β- lactam moiety from the C1-pool," Helv., vol. 70, pp. 412-420, 1987.
 D. Harzallah, and L. Larous, "The synthesis of tabtoxin peptide bond in Pseudomonas syringae pv. tabaci," Biochemical Society Transactions, vol. 26, no. 4, pp. 5383, Nov. 1998.
 P. A. Taylor, and R. D. Durbin, "The production and proprieties of chlorosis inducing toxins from a pseudomonad attacking timothy," Physiol. Plant Pathol., vol. 3, no. 1, pp. 9-17, Jan. 1973.