%0 Journal Article
	%A Hong Ding and  Fatiha Benslimane and  Isra Marei and  Chris R. Triggle
	%D 2012
	%J International Journal of Pharmacological and Pharmaceutical Sciences
	%B World Academy of Science, Engineering and Technology
	%I Open Science Index 64, 2012
	%T High Glucose Increases Acetylcholine-Induced Ca2+ Entry and Protein Expression of STIM1
	%U https://publications.waset.org/pdf/8247
	%V 64
	%X Hyperglycaemia is a key factor that contributes to the
development of diabetes-related microvascular disease and a major
risk factor for endothelial dysfunction. In the current study, we have
explored glucose-induced abnormal intracellular calcium (Ca2+
i)
homeostasis in mouse microvessel endothelial cells (MMECs) in
high glucose (HG) (40mmol/L) versus control (low glucose, LG) (11
mmol/L). We demonstrated that the exposure of MMECs to HG for 3
days did not change basal Ca2+
i, however, there was a significant
increase of acetylcholine-induced Ca2+ entry. Western blots
illustrated that exposure to HG also increased STIM1 (Stromal
Interaction Molecule 1), but not Orai1 (the pore forming subunit),
protein expression levels. Although the link between HG-induced
changes in STIM1 expression, enhanced Ca2+ entry and endothelial
dysfunction requires further study, the current data are suggestive
that targeting these pathways may reduce the impact of HG on
endothelial function.
	%P 67 - 70