@article{(Open Science Index):https://publications.waset.org/pdf/8247, title = {High Glucose Increases Acetylcholine-Induced Ca2+ Entry and Protein Expression of STIM1}, author = {Hong Ding and Fatiha Benslimane and Isra Marei and Chris R. Triggle}, country = {}, institution = {}, abstract = {Hyperglycaemia is a key factor that contributes to the development of diabetes-related microvascular disease and a major risk factor for endothelial dysfunction. In the current study, we have explored glucose-induced abnormal intracellular calcium (Ca2+ i) homeostasis in mouse microvessel endothelial cells (MMECs) in high glucose (HG) (40mmol/L) versus control (low glucose, LG) (11 mmol/L). We demonstrated that the exposure of MMECs to HG for 3 days did not change basal Ca2+ i, however, there was a significant increase of acetylcholine-induced Ca2+ entry. Western blots illustrated that exposure to HG also increased STIM1 (Stromal Interaction Molecule 1), but not Orai1 (the pore forming subunit), protein expression levels. Although the link between HG-induced changes in STIM1 expression, enhanced Ca2+ entry and endothelial dysfunction requires further study, the current data are suggestive that targeting these pathways may reduce the impact of HG on endothelial function.}, journal = {International Journal of Pharmacological and Pharmaceutical Sciences}, volume = {6}, number = {4}, year = {2012}, pages = {67 - 70}, ee = {https://publications.waset.org/pdf/8247}, url = {https://publications.waset.org/vol/64}, bibsource = {https://publications.waset.org/}, issn = {eISSN: 1307-6892}, publisher = {World Academy of Science, Engineering and Technology}, index = {Open Science Index 64, 2012}, }