@article{(Open Science Index):https://publications.waset.org/pdf/8093,
	  title     = {Codon-optimized Carbonic Anhydrase from Dunaliella species: Expression and Characterization},
	  author    = {Seung Pil Pack},
	  country	= {},
	  institution	= {},
	  abstract     = {Carbonic anhydrases (CAs) has been focused as
biological catalysis for CO2 sequestration process because it can
catalyze the conversion of CO2 to bicarbonate. Here, codon-optimized
sequence of α type-CA cloned from Duneliala species. (DsCAopt) was
constructed, expressed, and characterized. The expression level in E.
coli BL21(DE3) was better for codon-optimized DsCAopt than intact
sequence of DsCAopt. DsCAopt enzyme shows high-stability at pH
7.6/10.0. In final, we demonstrated that in the Ca2+ solution, DsCAopt
enzyme can catalyze well the conversion of CO2 to CaCO3, as the
calcite form.},
	    journal   = {International Journal of Biotechnology and Bioengineering},
	  volume    = {6},
	  number    = {8},
	  year      = {2012},
	  pages     = {603 - 607},
	  ee        = {https://publications.waset.org/pdf/8093},
	  url   	= {https://publications.waset.org/vol/68},
	  bibsource = {https://publications.waset.org/},
	  issn  	= {eISSN: 1307-6892},
	  publisher = {World Academy of Science, Engineering and Technology},
	  index 	= {Open Science Index 68, 2012},
	}