The Oxidative Stress and the Antioxidant Defense of the Lower Vegetables towards an Environmental Pollution

Authors: Fadila Khaldi, Nedjoud Grara, Houria Berrebbah, Mohamed Réda Djebar

Abstract:

The use of bioindicators plants (lichens, bryophytes and Sphagnum....) in monitoring pollution by heavy metals has been the subject of several works. However, few studies have addressed the impact of specific type-s pollutants (fertilizers, pesticides.) on these organisms. We propose in this work to make the highlighting effect of NPKs (NPK: nitrogen-phosphate-potassium-sulfate (NP2O5K2O) (15,15,15), at concentrations of 10, 20, 30 , 40 and 50mM/L) on the activity of detoxification enzymes (GSH/GST, CAT, APX and MDA) of plant bioindicators (mosses and lichens) after treatment for 3 and 7 days. This study shows the important role of the defense system in the accumulation and tolerance to chemical pollutants through the activation of enzymatic (GST (glutathione-S-transferase, APX (ascorbat peroxidase), CAT (catalase)) and nonenzymatic biomarkers (GSH (glutathione), MDA (malondialdehyde)) against oxidative stress generated by the NPKs.

Keywords: NPKs, Bioindicators, lower plants, GSH / GST, CAT, APX and MDA.

Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1329076

References:

[1] Laurent B et Ahmed B.(1991). La germination des semences en conditions sèches. Sciences et changements planétaires/Sécheresse 2(4) :239-249.
[2] Chambers PA., Guy M., Roberts E., Charlton MN., Kent R., Gagnon C., Grove G. et Foster N. (2001). Les éléments nutritifs et leurs effets sur l-environnement. Agriculture et Agroalimentaire, Environnement Canada, P├¬ches et Océans Canada, Santé Canada et Ressources naturelles Canada, Ottawa.
[3] Leclerc J.C. (1999). Ecophysiologie végétale. Publication de l-université de Saint-Etienne :171-180pages.
[4] Garrec J-P et Van Haluwyn C. (2002) . Biosurveillance végétale de la qualité de l-air : concepts , méthodes et applications. Paris, TEC & DOC Eds.
[5] Khaldi F. (2003). Toxicité du nitrate d-ammonium NH4NO3 sur trois modèles biologiques : les paramécies, les mousses et les lichens. Effet sur leur métabolisme respiratoire. Mémoire de Magister en Biochimie Appliquée. Université Badji Mokhtar, Annaba : 86pages.
[6] Ozenda, P., 2000. Les végétaux, organisation et diversité biologique 2eme Edition: Masson: pp: 192.
[7] Jahns, H.M., 1989. Guide des fougères, Mousses et Lichens d-Europe. Edition Delachaux et Nestlé.pp: 224.
[8] Bensoltane, S., F. Khaldi, H. Djebar and M.R. Djebar, 2005. Toxicity of the ammonium nitrate NH NO on the respiratory metabolism of three biological models: Parmeciums, mosses and lichens. Comm. Appl. Biol. Sci. Ghent University,70/4: 1043-1051.
[9] Cakmak I et Horst W.J. (1991). Effect of aluminium on lipid peroxidation, superoxide dismutase, catalase and peroxidase activities in root tips of soybean (glycine max).Physiol.Plant 83:463-468.
[10] Nakano Y and Azada K. (1987). Purification of ascorbate peroxidase in spinach chloroplasts: its inactivation in ascorbate depleted medium and reactivation by monodehydroascorbate radical.Plant Cell Physiol.28:131-140.
[11] Weckberker G, and Cory G. (1988). Ribonucléotide reductase activity abd growth of glutathione depleted mouse leukemial 1210 cells in vitro. Cacer letters 40: 257-264.
[12] Habig W.H , Pabst M.J, Jakoby W.B. (1974). Glutathione Stransferases. J. Biol.Chem. 249, 7130- 7139.
[13] Bradford M M. (1976 ). A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal.Biochem.72:248-254.
[14] Alia, Prasad ,K.V.S.K.,Pardha Saradhi P.(1995). Effect of zinc on free radical and proline in Brassica juncea and Cajanus cajan. Phytochemestry 39,45-47.
[15] Dagnelie P.(1999).Statistiques théoriques et appliquées. Tome 2 : références statistiques ├á une et ├á deux dimensions. Bruxelles. Univ De BOECK et LARCIER, 659 p.
[16] Brunialti G. and Frati L. (2007). Biomonitoring of nine elements by the lichen Xanthoria parietina in Adriatic Italy: a retrospective study over a 7-year time span, The Science of the Total Environment, 387,p :289-300.
[17] Smirnoff, N. (1998). Plant resistance to environmental stress. Curr. Opin. Biotechnol.9: 214-219.
[18] Nimptsch J and Pflugmacher S. (2007). Ammonia triggers the promotion of oxidative stress in the aquatic macrophyte Myriophyllum mattogrossense.Chemosphere 66 :708-714.
[19] Asada K . and Takahashi M . (1987). Production and scavenging of active oxygen in photosynthesis. In: Kyle DJ, Osmond CJ, Arntzen CJ(Eds), Photo inhibition: topics in Photosynthesis .Elsevier , Amsterdam.:227-231.
[20] Zhu YL, Pilon-Smits E, Tarun AS, Jouanin L, Terry N .(1999). Overex pression of glutathione synthetase in indian mustard enhances cadmium accumulation and tolerance. Plant Physiology 119: 73-80.
[21] Cao X, Ma L.Q., Tu C.(2004). Antioxydative reponses to arsenic in the arsenic hyperaccumulator Chinese brake fern environ.Pollut. 128, 317- 325.
[22] Freeman JL, Persans MW, Nieman K, Albrecht C, Peer W, Pickering I, Salt DE .( 2004). Increased glutathione biosynthesis plays a role in nickel tolerance in Thlaspi nickel hyperaccumulators. Plant Cell 16: 2176-2191.
[23] Gallego S.M, Benavides M.P and Tomaro M.L. (1996). Effect of heavy Metal ion excess on sunflower leaves: evidence for involvement of an oxidative stress.Plant Sci 121: 151- 159.
[24] Nagalakshmi N and Prasad M.N.V. (2001). Reponses of glutathione cycle enzymes and glutathione metabolism to copper stress in Scenedesmus bijugatus. Plant Sci 160: 291-299.
[25] Durcuix C, Junot C, Fiévert J.B ,Villiers F, Ezan E and Bourguignon J .(2006).New insights into the régulation of phytochelation biosynthesis in A.thaliana cells from metabolite profiling analyses. Biochimie 88 :1733-1742.
[26] Per┼íić A. (2004) . Modalités de contamination par les polluants organiques persistants des réseaux trophiques lagunaires. Application de la méthode des isotopes stables .Thèse de Doctorat. Université Paris XI. p.159.
[27] Lagadic L, Caquet T, Amiard J.C. (1997). Biomarqueurs en écotoxicologie.Aspects fondamentaux. Ed Masson 196p.
[28] Apel K and Hirt H. (2004). Reactive oxygen species: metabolism, oxidative stress, and signal transduction. Annu. Rev. Plant Biol. 55: 373-399.
[29] Modenesi P .(1993). An SEM study of injury symptoms in Parmotremareticulatum treated with paraquat or growing in sulphur dioxide-polluted air. Lichenologist 25 :423-433.
[30] Loewus F.A .(1998). Ascorbic acid and its metabolic products. In The Biochemestry of plants, Vol.14(J.Preiss,ed.):85-107. Nrw York:Academic Press.
[31] Farago E.M.(1994). Plant and Chemical Elements : Biochemestry,Uptake,Tolerance and Toxicity Ed.VCH32-231.
[32] Mittler R,Vanderauwera S, Gollery M, Van Breusegem F.(2004). Réactive oxygen gene network of plants. Tends Plant Sci 9 :490-498.
[33] Foyer CH, Descourvières P, Kunert KJ. (1994a). Protection against oxygen radicals: an important defence mechanism studied in transgenic plants. Plant Cell. Env. 17, p. 507-523.
[34] Sinha S, Saxena R and Singh S. (2005). Chromium induced lipid peroxidation in the plants of Pistia stratiotes L.: role of antioxidants and antioxidant enzymes.Chemosphere 58, 595-604.
[35] Mishra S, Srivastava S, Tripathi R.D, Govindarajan R, Kuriakose S.V, Prasad M.N.V. (2006a). Phytochelatin synthesis and response of antioxidants during cadmium stress in Bacopa monnieri L. Plant Physiol. Biochem. 44, 25-37.
[36] Mishra S, Srivastava S, Tripathi R.D, Kumar R, Seth C.S, Gupta D.K. (2006b). Lead detoxification by coontail (Ceratophyllum demersum L.) involves induction of phytochelatins and antioxidant system in response to its accumulation.Chemosphere 65, 1027-1039.
[37] Dazy M, Masfaraud J.F et Férard J.F. (2009). Induction of oxidative stress biomarkers associated with heavy metal stress in Fontinalis antipyretica Hedw.Elsevier. Chemosphere 75 : 297-302.
[38] Narbonne J.F, Garrigues P, Ribera D, Raoux C, Mathieu A, Lemaire P,Salaun J.P, Lafaurie M. (1991). Mixed-function oxygenase enzymes as tools for pollution monitoring : field studies on the French coast of the Mediterranean sea.Comp. Biochem. Physiol., 100C, 37-42.
[39] Sairam RK , Srivastava GC. (2002). Changes in antioxidant activity in sub-cellular fraction of tolerant and susceptible wheat genotypes in response to long term salt stress. Plant Sci. 162: 897-904.
[40] Xue T, Hartikaienen H, Piironen V. (2001). Antioxidative and growthpromoting effect of selenium on senescing lettuce. Plant Oil 237, p. 55- 61.
[41] De Vos C.H.R, Bookum V.M.T, Vooijs R, Schat H and De Kok L.J.(1993). Effect of copper on fatty acid composition and peroxidation of lipids in roots of copper tolerant and sensitive Silene cucubalus. Plant. Physiol. Biochem. 31(2): 151-158.
[42] Weckx J.E.J and Clijsters H.M.M. (1996). Oxidative damage and defense mechanisms in primary leaves of phaseolus vulgaris as a result of root assimilation of toxic amounts of copper. Physiologia Plantarum. 96 : 506-512.