TY - JFULL AU - M. Petchsangsai and N. Wonglertnirant and T. Rojanarata and P. Opanasopit and T. Ngawhirunpat PY - 2012/10/ TI - Microneedles-Mediated Transdermal Delivery T2 - International Journal of Pharmacological and Pharmaceutical Sciences SP - 438 EP - 442 VL - 6 SN - 1307-6892 UR - https://publications.waset.org/pdf/4890 PU - World Academy of Science, Engineering and Technology NX - Open Science Index 69, 2012 N2 - The objective of the present study was to evaluate the potential of hollow microneedles for enhancing the transdermal delivery of Bovine Serum Albumin (MW~66,000 Da)-Fluorescein Isothiocyanate (BSA-FITC) conjugate, a hydrophilic large molecular compound. Moreover, the effect of different formulations was evaluated. The series of binary mixtures composed of propylene glycol (PG) and pH 7.4 phosphate buffer solution (PBS) was prepared and used as a medium for BSA-FITC. The results showed that there was no permeation of BSA-FITC solution across the neonatal porcine skin without using hollow microneedles, whereas the cumulative amount of BSA-FITC released at 8 h through the neonatal porcine skin was about 60-70% when using hollow microneedles. Furthermore, the results demonstrated that the higher volume of PG in binary mixtures injected, the lower cumulative amount of BSA-FITC released and release rate of BSA-FITC from skin. These release profiles of BSA-FITC in binary mixtures were expressed by Fick-s law of diffusion. These results suggest the utilization of hollow microneedle to enhance transdermal delivery of protein and provide useful information for designing an effective hollow microneedle system. ER -