%0 Journal Article
	%A T. Sadunishvili and  E. Kvesitadze and  M. Betsiashvili and  N. Kuprava and  G. Zaalishvili and  G. Kvesitadze
	%D 2009
	%J International Journal of Biomedical and Biological Engineering
	%B World Academy of Science, Engineering and Technology
	%I Open Science Index 33, 2009
	%T Influence of Hydrocarbons on Plant Cell Ultrastructure and Main Metabolic Enzymes
	%U https://publications.waset.org/pdf/4869
	%V 33
	%X Influence of octane and benzene on plant cell
ultrastructure and enzymes of basic metabolism, such as nitrogen
assimilation and energy generation have been studied. Different
plants: perennial ryegrass (Lolium perenne) and alfalfa (Medicago
sativa); crops- maize (Zea mays L.) and bean (Phaseolus vulgaris);
shrubs – privet (Ligustrum sempervirens) and trifoliate orange
(Poncirus trifoliate); trees - poplar (Populus deltoides) and white
mulberry (Morus alba L.) were exposed to hydrocarbons of different
concentrations (1, 10 and 100 mM). Destructive changes in bean and
maize leaves cells ultrastructure under the influence of benzene
vapour were revealed at the level of photosynthetic and energy
generation subcellular organells. Different deviations at the level of
subcellular organelles structure and distribution were observed in
alfalfa and ryegrass root cells under the influence of benzene and
octane, absorbed through roots. The level of destructive changes is
concentration dependent. Benzene at low 1 and 10 mM concentration
caused the increase in glutamate dehydrogenase (GDH) activity in
maize roots and leaves and in poplar and mulberry shoots, though to
higher extent in case of lower, 1mM concentration. The induction
was more intensive in plant roots. The highest tested 100mM
concentration of benzene was inhibitory to the enzyme in all plants.
Octane caused induction of GDH in all grassy plants at all tested
concentrations; however the rate of induction decreased parallel to
increase of the hydrocarbon concentration. Octane at concentration 1
mM caused induction of GDH in privet, trifoliate and white mulberry
shoots. The highest, 100mM octane was characterized by inhibitory
effect to GDH activity in all plants. Octane had inductive effect on
malate dehydrogenase in almost all plants and tested concentrations,
indicating the intensification of Trycarboxylic Acid Cycle.
The data could be suggested for elaboration of criteria for plant
selection for phytoremediation of oil hydrocarbons contaminated
soils.
	%P 429 - 434