R. Sullivan and T. Holden and G. Tremberger and Jr and E. Cheung and C. Branch and J. Burrero and G. Surpris and S. Quintana and A. Rameau and N. Gadura and H. Yao and R. Subramaniam and P. Schneider and S. A. Rotenberg and P. Marchese and A. Flamhlolz and D. Lieberman and T. Cheung
Fractal Dimension of Breast Cancer Cell Migration in a Wound Healing Assay
186 - 191
2008
2
8
International Journal of Biomedical and Biological Engineering
https://publications.waset.org/pdf/4794
https://publications.waset.org/vol/20
World Academy of Science, Engineering and Technology
Migration in breast cancer cell wound healing assay
had been studied using image fractal dimension analysis. The
migration of MDAMB231 cells (highly motile) in a wound healing
assay was captured using timelapse phase contrast video microscopy
and compared to MDAMB468 cell migration (moderately motile).
The Higuchi fractal method was used to compute the fractal
dimension of the image intensity fluctuation along a single pixel
width region parallel to the wound. The nearwound region fractal
dimension was found to decrease three times faster in the MDAMB
231 cells initially as compared to the less cancerous MDAMB468
cells. The inner region fractal dimension was found to be fairly
constant for both cell types in time and suggests a wound influence
range of about 15 cell layer. The boxcounting fractal dimension
method was also used to study region of interest (ROI). The MDAMB
468 ROI area fractal dimension was found to decrease
continuously up to 7 hours. The MDAMB231 ROI area fractal
dimension was found to increase and is consistent with the behavior
of a HGFtreated MDAMB231 wound healing assay posted in the
public domain. A fractal dimension based capacity index has been
formulated to quantify the invasiveness of the MDAMB231 cells in
the perpendiculartowound direction. Our results suggest that image
intensity fluctuation fractal dimension analysis can be used as a tool
to quantify cell migration in terms of cancer severity and treatment
responses.
Open Science Index 20, 2008