TY - JFULL AU - Hanina M. N. and Hairul Shahril M. and Mohd Fazrullah Innsan M. F. and Ismatul Nurul Asyikin I. and Abdul Jalil A. K and Salina M. R. and Ahmad I.B. PY - 2011/12/ TI - Protein Production by Bacillus Subtilis Atcc 21332 in the Presence of Cymbopogon Essential Oils T2 - International Journal of Biotechnology and Bioengineering SP - 780 EP - 785 VL - 5 SN - 1307-6892 UR - https://publications.waset.org/pdf/4609 PU - World Academy of Science, Engineering and Technology NX - Open Science Index 59, 2011 N2 - Proteins levels produced by bacteria may be increased in stressful surroundings, such as in the presence of antibiotics. It appears that many antimicrobial agents or antibiotics, when used at low concentrations, have in common the ability to activate or repress gene transcription, which is distinct from their inhibitory effect. There have been comparatively few studies on the potential of antibiotics or natural compounds in nature as a specific chemical signal that can trigger a variety of biological functions. Therefore, this study was focusing on the effect of essential oils from Cymbopogon flexuosus and C. nardus in regulating proteins production by Bacillus subtilis ATCC 21332. The Minimum Inhibition Concentrations (MICs) of both essential oils on B. subtilis were determined by using microdilution assay, resulting 0.2% and 1.56% for each C. flexuosus and C. nardus subsequently. The bacteria were further exposed to each essential oils at concentration of 0.01XMIC for 2 days. The proteins were then isolated and analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Protein profile showed that a band with approximate size of 250 kD was appeared for the treated bacteria with essential oils. Thus, Bacillus subtilis ATCC 21332 in stressful condition with the presence of essential oils at low concentration could induce the protein production. ER -