Y. Baba hamed and A. Medjdoub and H. Merzouk and M. Narce
Changes of in vitro Cytokine Production induced by δLactams
699 - 703
2012
6
8
International Journal of Chemical and Molecular Engineering
https://publications.waset.org/pdf/4123
https://publications.waset.org/vol/68
World Academy of Science, Engineering and Technology
The aim of this work was to study the in vitro effects
of δlactam 1 and its 4chlorophenyl derivative 2, on the proliferative
responses of human lymphocytes and Th1 and Th2 cytokine
secretion. The possible protective role of vitamin E on intracellular
stress oxidative induced by these compounds was also investigated.
Peripheral blood lymphocytes were isolated using differential
centrifugation on a density gradient of Histopaque. They were
cultured with mitogen concanavalin A, vitamin E (10 μM) and with
different concentrations of the compounds 1 and 2 (0.1 to 10 μM).
Proliferation (MTT assay), IL2, INFγ and IL4 (Elisa kits),
intracellular superoxide anion were determined. 1 and 2 were
immunostimulant and increased cytokine secretion with a shift away
from Th1 response to Th2. These properties were however
accompanied by an increase in intracellular oxidative stress. The
presence of vitamin E exhibited protective effects by reducing δ
lactaminduced superoxide anion generation in lymphocytes.
Open Science Index 68, 2012