%0 Journal Article
	%A Y. Baba hamed and  A. Medjdoub and  H. Merzouk and  M. Narce
	%D 2012
	%J International Journal of Chemical and Molecular Engineering
	%B World Academy of Science, Engineering and Technology
	%I Open Science Index 68, 2012
	%T Changes of in vitro Cytokine Production induced by δ-Lactams
	%U https://publications.waset.org/pdf/4123
	%V 68
	%X The aim of this work was to study the in vitro effects
of δ-lactam 1 and its 4-chlorophenyl derivative 2, on the proliferative
responses of human lymphocytes and Th1 and Th2 cytokine
secretion. The possible protective role of vitamin E on intracellular
stress oxidative induced by these compounds was also investigated.
Peripheral blood lymphocytes were isolated using differential
centrifugation on a density gradient of Histopaque. They were
cultured with mitogen concanavalin A, vitamin E (10 μM) and with
different concentrations of the compounds 1 and 2 (0.1 to 10 μM).
Proliferation (MTT assay), IL-2, INFγ and IL-4 (Elisa kits),
intracellular superoxide anion were determined. 1 and 2 were
immunostimulant and increased cytokine secretion with a shift away
from Th1 response to Th2. These properties were however
accompanied by an increase in intracellular oxidative stress. The
presence of vitamin E exhibited protective effects by reducing δ-
lactam-induced superoxide anion generation in lymphocytes.
	%P 693 - 697