@article{(Open Science Index):https://publications.waset.org/pdf/4123, title = {Changes of in vitro Cytokine Production induced by δ-Lactams}, author = {Y. Baba hamed and A. Medjdoub and H. Merzouk and M. Narce}, country = {}, institution = {}, abstract = {The aim of this work was to study the in vitro effects of δ-lactam 1 and its 4-chlorophenyl derivative 2, on the proliferative responses of human lymphocytes and Th1 and Th2 cytokine secretion. The possible protective role of vitamin E on intracellular stress oxidative induced by these compounds was also investigated. Peripheral blood lymphocytes were isolated using differential centrifugation on a density gradient of Histopaque. They were cultured with mitogen concanavalin A, vitamin E (10 μM) and with different concentrations of the compounds 1 and 2 (0.1 to 10 μM). Proliferation (MTT assay), IL-2, INFγ and IL-4 (Elisa kits), intracellular superoxide anion were determined. 1 and 2 were immunostimulant and increased cytokine secretion with a shift away from Th1 response to Th2. These properties were however accompanied by an increase in intracellular oxidative stress. The presence of vitamin E exhibited protective effects by reducing δ- lactam-induced superoxide anion generation in lymphocytes.}, journal = {International Journal of Chemical and Molecular Engineering}, volume = {6}, number = {8}, year = {2012}, pages = {699 - 703}, ee = {https://publications.waset.org/pdf/4123}, url = {https://publications.waset.org/vol/68}, bibsource = {https://publications.waset.org/}, issn = {eISSN: 1307-6892}, publisher = {World Academy of Science, Engineering and Technology}, index = {Open Science Index 68, 2012}, }