Aree Thongpukdee and Ekasit Nisayan and Chockpisit Thepsithar
Multiple Shoot Formation of Paphiopedilum &039;Delrosi&039;
399 - 402
2013
7
6
International Journal of Bioengineering and Life Sciences
https://publications.waset.org/pdf/3966
https://publications.waset.org/vol/78
World Academy of Science, Engineering and Technology
Shoots, with three leaves, of Paphiopedilum &039;Delrosi&039;
were used as explants for multiple shoot induction. Modified
Hyponex medium was supplemented with thidiazuron (TDZ), N6
benzyladenine (BA) or kinetin (Kn) alone and in combinations with
2,4dichlorophenoxyacetic acid (2,4D). All explants were cultured
for 15 weeks. It was found that TDZ alone at the concentration of
0.45μM or in combination with 4.52μM 2,4D and 8.88μM BA in
combination with 13.56μM 2,4D promoted multiple shoots. The
highest shoot sprouting efficiencies (80.0, 90.0 and 80.0) and new
shoot numbers (1.5, 1.3 and 1.1) were obtained, respectively. Fresh
weight, height, numbers of leaf and root of new shoots and initial
explants were discussed.
Open Science Index 78, 2013