TY - JFULL AU - Aree Thongpukdee and Ekasit Nisayan and Chockpisit Thepsithar PY - 2013/7/ TI - Multiple Shoot Formation of Paphiopedilum 'Delrosi' T2 - International Journal of Bioengineering and Life Sciences SP - 398 EP - 402 VL - 7 SN - 1307-6892 UR - https://publications.waset.org/pdf/3966 PU - World Academy of Science, Engineering and Technology NX - Open Science Index 78, 2013 N2 - Shoots, with three leaves, of Paphiopedilum 'Delrosi' were used as explants for multiple shoot induction. Modified Hyponex medium was supplemented with thidiazuron (TDZ), N6- benzyladenine (BA) or kinetin (Kn) alone and in combinations with 2,4-dichlorophenoxyacetic acid (2,4-D). All explants were cultured for 15 weeks. It was found that TDZ alone at the concentration of 0.45μM or in combination with 4.52μM 2,4-D and 8.88μM BA in combination with 13.56μM 2,4-D promoted multiple shoots. The highest shoot sprouting efficiencies (80.0, 90.0 and 80.0%) and new shoot numbers (1.5, 1.3 and 1.1) were obtained, respectively. Fresh weight, height, numbers of leaf and root of new shoots and initial explants were discussed. ER -