%0 Journal Article
	%A Priyanka Gupta and  Paul Verma and  Kerry Hourigan and  Jayesh Bellare and  Sameer Jadhav
	%D 2012
	%J International Journal of Biotechnology and Bioengineering
	%B World Academy of Science, Engineering and Technology
	%I Open Science Index 72, 2012
	%T Metabolic Analysis of Fibroblast Conditioned Media and Comparison with Theoretical Modeling
	%U https://publications.waset.org/pdf/367
	%V 72
	%X Understanding the consumption and production of
various metabolites of fibroblast conditioned media is needed for its
proper and optimized use in expansion of pluripotent stem cells. For
this purpose, we have used the HPLC method to analyse the
consumption of glucose and the production of lactate over time by
mouse embryonic fibroblasts. The experimental data have also been
compared with mathematical model fits. 0.025 moles of lactate was
produced after 72 hrs while the glucose concentration decreased from
0.017 moles to 0.011 moles. The mathematical model was able to
predict the trends of glucose consumption and lactate production.
	%P 1070 - 1074