@article{(Open Science Index):https://publications.waset.org/pdf/3174,
	  title     = {Sterilisation of Hyponex Medium by Chemicals without Autoclaving and Growth of Phalaenopsis Protocorms},
	  author    = {Chockpisit Thepsithar and  Aree Thongpukdee},
	  country	= {},
	  institution	= {},
	  abstract     = {For sterilization of Phalaenopsis culture medium without autoclaving, selected single sterilizing agents and in combinations were added to a 25ml Hyponex medium in a 120ml glass container. Treated liquid and solid media, supplemented with sterilizing agents, were compared to a control medium, autoclaved at 121°C for 15min. It was found that 90(L of 10% povidone-iodine, 150(L of 5.25% sodium hypochlorite, 150(L of 2% mercurochrome, 90(L of 2.5% iodine + 2.5% potassium iodine in combination with 10% providone-iodine (1:3) and 30(L of 2.5% iodine + 2.5% potassium iodide in combination with 2% mercurochrome showed 100% sterile conditions in liquid medium but provided 75, 100, 50, 75 and 80% sterile conditions, respectively, in solid medium. For growth of Phalaenopsis protocorms, 90(L of 10% povidone-iodine in liquid Hyponex medium gave the comparable growth of protocorms to control medium while 150(L of 5.25% sodium hypochlorite in solid medium provided the promising growth of protocorms. Growth of protocorms, whole fresh weight, numbers of leaf and root, root length and number of protocorm-like bodies, was discussed.
},
	    journal   = {International Journal of Bioengineering and Life Sciences},
	  volume    = {7},
	  number    = {6},
	  year      = {2013},
	  pages     = {403 - 406},
	  ee        = {https://publications.waset.org/pdf/3174},
	  url   	= {https://publications.waset.org/vol/78},
	  bibsource = {https://publications.waset.org/},
	  issn  	= {eISSN: 1307-6892},
	  publisher = {World Academy of Science, Engineering and Technology},
	  index 	= {Open Science Index 78, 2013},
	}