%0 Journal Article %A Chun-Yen Sung and Chung-Yao Yang and Tzu-Chun Liao and Wen-Shiang Chen and Chao-Min Cheng and J. Andrew Yeh %D 2013 %J International Journal of Biomedical and Biological Engineering %B World Academy of Science, Engineering and Technology %I Open Science Index 74, 2013 %T Neuroblasts Micropatterning on Nanostructural Modified Chitosan Membranes %U https://publications.waset.org/pdf/2943 %V 74 %X The study describes chitosan membrane platform modified with nanostructure pattern which using nanotechnology to fabricate. The cell-substrate interaction between neuro-2a neuroblasts cell lines and chitosan membrane (flat, nanostructure and nanostructure pattern types) was investigated. The adhered morphology of neuro-2a cells depends on the topography of chitosan surface. We have found that neuro-2a showed different morphogenesis when cells adhered on flat and nanostructure chitosan membrane. The cell projected area of neuro-2a on flat chitosan membrane is larger than on nanostructure chitosan membrane. In addition, neuro-2a cells preferred to adhere on flat chitosan surface region than on nanostructure chitosan membrane to immobilize and differentiation. The experiment suggests surface topography can be used as a critical mechanism to isolate group of neuro-2a to a particular rectangle area on chitosan membrane. Our finding will provide a platform to take patch clamp to record electrophysiological behavior about neurons in vitro in the future. %P 91 - 94