%0 Journal Article
	%A Yan Ren and  Jun Qiao and  Xianxia Liu and  Pengyan Wang and  Qiang Fu and  Huijun Shi and  Fei Guo and  Yuanzhi Wang and  Hui Zhang and  Jinliang Sheng and  Xinli Gu and  Xiao-Jun Liu and  Chuangfu Chen
	%D 2013
	%J International Journal of Biotechnology and Bioengineering
	%B World Academy of Science, Engineering and Technology
	%I Open Science Index 75, 2013
	%T Molecular Epidemiology and Genotyping of Bovine Viral Diarrhea Virus in Xinjiang Uygur Autonomous Region of China
	%U https://publications.waset.org/pdf/2644
	%V 75
	%X As part of national epidemiological survey on bovine
viral diarrhea virus (BVDV), a total of 274 dejecta samples were
collected from 14 cattle farms in 8 areas of Xinjiang Uygur
Autonomous Region in northwestern China. Total RNA was extracted
from each sample, and 5--untranslated region (UTR) of BVDV
genome was amplified by using two-step reverse
transcriptase-polymerase chain reaction (RT-PCR). The PCR products
were subsequently sequenced to study the genetic variations of BVDV
in these areas. Among the 274 samples, 33 samples were found
virus-positive. According to sequence analysis of the PCR products,
the 33 samples could be arranged into 16 groups. All the sequences,
however, were highly conserved with BVDV Osloss strains. The virus
possessed theses sequences belonged to BVDV-1b subtype by
phylogenetic analysis. Based on these data, we established a typing
tree for BVDV in these areas. Our results suggested that BVDV-1b
was a predominant subgenotype in northwestern China and no
correlation between the genetic and geographical distances could be
observed above the farm level.
	%P 234 - 238