Hong Yi Huang and Wei Ti Kuo and Yi You Huang
Combining Gene and Chemo Therapy using Multifunctional Polymeric Micelles
240 - 243
2010
4
5
International Journal of Biomedical and Biological Engineering
https://publications.waset.org/pdf/2612
https://publications.waset.org/vol/41
World Academy of Science, Engineering and Technology
Nonviral gene carriers composed of biodegradable
polymers or lipids have been considered as a safer alternative for gene
carriers over viral vectors. We have developed multifunctional
nanomicelles for both drug and gene delivery application.
Polyethyleneimine (PEI) was modified by grafting stearic acid (SA)
and formulated to polymeric micelles (PEISA) with positive surface
charge for gene and drug delivery. Our results showed that PEISA
micelles provided high siRNA binding efficiency. In addition, siRNA
delivered by PEISA carriers also demonstrated significantly high
cellular uptake even in the presence of serum proteins. The
posttranscriptional gene silencing efficiency was greatly improved by
the polyplex formulated by 10k PEISAsiRNA. The amphiphilic
structure of PEISA micelles provided advantages for multifunctional
tasks; where the hydrophilic shell modified with cationic charges can
electrostatically interact with DNA or siRNA, and the hydrophobic
core can serve as payloads for hydrophobic drugs, making it a
promising multifunctional vehicle for both genetic and chemotherapy
application.
Open Science Index 41, 2010