%0 Journal Article
	%A Masaki Yamaguchi and  Akira Ito and  Noriaki Okamoto and  Yoshinori Kawabe and  Masamichi Kamihira
	%D 2012
	%J International Journal of Biotechnology and Bioengineering
	%B World Academy of Science, Engineering and Technology
	%I Open Science Index 71, 2012
	%T A Heat-Inducible Transgene Expression System for Gene Therapy
	%U https://publications.waset.org/pdf/2563
	%V 71
	%X Heat-inducible gene expression vectors are useful for hyperthermia-induced cancer gene therapy, because the combination
of hyperthermia and gene therapy can considerably improve the therapeutic effects. In the present study, we developed an enhanced
heat-inducible transgene expression system in which a heat-shock
protein (HSP) promoter and tetracycline-responsive transactivator
were combined. When the transactivator plasmid containing the
tetracycline-responsive transactivator gene was co-transfected with
the reporter gene expression plasmid, a high level of heat-induced gene expression was observed compared with that using the HSP
promoter without the transactivator. In vitro evaluation of the
therapeutic effect using HeLa cells showed that heat-induced therapeutic gene expression caused cell death in a high percentage of
these cells, indicating that this strategy is promising for cancer gene therapy.
	%P 1007 - 1010