@article{(Open Science Index):https://publications.waset.org/pdf/2563,
	  title     = {A Heat-Inducible Transgene Expression System for Gene Therapy},
	  author    = {Masaki Yamaguchi and  Akira Ito and  Noriaki Okamoto and  Yoshinori Kawabe and  Masamichi Kamihira},
	  country	= {},
	  institution	= {},
	  abstract     = {Heat-inducible gene expression vectors are useful for hyperthermia-induced cancer gene therapy, because the combination
of hyperthermia and gene therapy can considerably improve the therapeutic effects. In the present study, we developed an enhanced
heat-inducible transgene expression system in which a heat-shock
protein (HSP) promoter and tetracycline-responsive transactivator
were combined. When the transactivator plasmid containing the
tetracycline-responsive transactivator gene was co-transfected with
the reporter gene expression plasmid, a high level of heat-induced gene expression was observed compared with that using the HSP
promoter without the transactivator. In vitro evaluation of the
therapeutic effect using HeLa cells showed that heat-induced therapeutic gene expression caused cell death in a high percentage of
these cells, indicating that this strategy is promising for cancer gene therapy.},
	    journal   = {International Journal of Biotechnology and Bioengineering},
	  volume    = {6},
	  number    = {11},
	  year      = {2012},
	  pages     = {1007 - 1010},
	  ee        = {https://publications.waset.org/pdf/2563},
	  url   	= {https://publications.waset.org/vol/71},
	  bibsource = {https://publications.waset.org/},
	  issn  	= {eISSN: 1307-6892},
	  publisher = {World Academy of Science, Engineering and Technology},
	  index 	= {Open Science Index 71, 2012},