@article{(Open Science Index):https://publications.waset.org/pdf/2563, title = {A Heat-Inducible Transgene Expression System for Gene Therapy}, author = {Masaki Yamaguchi and Akira Ito and Noriaki Okamoto and Yoshinori Kawabe and Masamichi Kamihira}, country = {}, institution = {}, abstract = {Heat-inducible gene expression vectors are useful for hyperthermia-induced cancer gene therapy, because the combination of hyperthermia and gene therapy can considerably improve the therapeutic effects. In the present study, we developed an enhanced heat-inducible transgene expression system in which a heat-shock protein (HSP) promoter and tetracycline-responsive transactivator were combined. When the transactivator plasmid containing the tetracycline-responsive transactivator gene was co-transfected with the reporter gene expression plasmid, a high level of heat-induced gene expression was observed compared with that using the HSP promoter without the transactivator. In vitro evaluation of the therapeutic effect using HeLa cells showed that heat-induced therapeutic gene expression caused cell death in a high percentage of these cells, indicating that this strategy is promising for cancer gene therapy.}, journal = {International Journal of Biotechnology and Bioengineering}, volume = {6}, number = {11}, year = {2012}, pages = {1007 - 1010}, ee = {https://publications.waset.org/pdf/2563}, url = {https://publications.waset.org/vol/71}, bibsource = {https://publications.waset.org/}, issn = {eISSN: 1307-6892}, publisher = {World Academy of Science, Engineering and Technology}, index = {Open Science Index 71, 2012}, }