Performance Evaluation of an Amperometric Biosensor using a Simple Microcontroller based Data Acquisition System

Authors: V. G. Sangam, Balasaheb M. Patre

Abstract:

In this paper we have proposed a methodology to develop an amperometric biosensor for the analysis of glucose concentration using a simple microcontroller based data acquisition system. The work involves the development of Detachable Membrane Unit (enzyme based biomembrane) with immobilized glucose oxidase on the membrane and interfacing the same to the signal conditioning system. The current generated by the biosensor for different glucose concentrations was signal conditioned, then acquired and computed by a simple AT89C51-microcontroller. The optimum operating parameters for the better performance were found and reported. The detailed performance evaluation of the biosensor has been carried out. The proposed microcontroller based biosensor system has the sensitivity of 0.04V/g/dl, with a resolution of 50mg/dl. It has exhibited very good inter day stability observed up to 30 days. Comparing to the reference method such as HPLC, the accuracy of the proposed biosensor system is well within ± 1.5%. The system can be used for real time analysis of glucose concentration in the field such as, food and fermentation and clinical (In-Vitro) applications.

Keywords: Biosensor, DMU, Glucose oxidase andMicrocontroller.

Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1056276

References:

[1] Chaubey A., Malhotra B. D., "Mediated biosensors", Biosensors and Bioelectronics 17, 2002, pp. 441-456.
[2] Guilbault G. G., ÔÇÿAnalytical uses of immobilized enzymes-, Mercal Dekkar, 1984, pp. 77-160.
[3] Wolfgang Gopel., and Peter Heiduschka., "Interface analysis in biosensor design", Biosensors and bioelectronics 10, 1995, pp. 853-883.
[4] Baronus R., Ivanauskas E., Kulys J., "Computer simulation of the response of Amperometric Biosensors in stirred and non stirred solution", Nonlinear Analysis: Modeling and control 8, 2003, pp. 3- 18.
[5] Rawson D. and Gaisford W, "Biosensors monitor the environment", Control Instrum 22, 1990, pp. 63-64.
[6] Bilitewski U., Jager A., Ruger P., and Weise W., "Enzyme electrodes for the determination of carbohydrates in food", Sensors and Actuators B, 1983, pp.113-118.
[7] Wohltjen H., "Chemical Micro sensors and Micro instrumentation", Anal.Chem. 56, 1984, pp. 87-103.
[8] Wilson G.S., Thevenot D. R., (Ed: A.G.E. Cass) "Biosensors a practical approach", Oxford University Press, 1990, pp. 5-6.
[9] Wilson R., and A. P.F. Turner, "Glucose oxidase an ideal enzyme", Biosensors and Bioelectronics 7, 1992, pp. 165-185.
[10] Sangam V. G., and Patre B. M., "Development of computer based amperometric biosensor for the analysis of glucose concentration", In Proc. International conference, SICE-Japan, Okayama University, Japan,2005 pp.105.
[11] Ashok Mulchandani and Kim Rogers, "Enzyme and Microbial Biosensors - Techniques and protocols", Human Press, Totowa, New Jersey, 1998, pp. 199-218.
[12] Wang J., Mulchandani A., Mulchandani P and Chen W, "Remote biosensor for in situ monitoring of organophosphate nerve agent", Electroanalysis, 11, 1999, pp. 866-869.
[13] Newman J., White S., Tothill I and Turner A. P. F., "Cataylic materials, membranes and fabrication technologies suitable for the construction of amperometric biosensors", Anal. Chem. 67, 1995, pp. 4594-4599.
[14] Willner I., and Riklin A, "Electrical communication between electrodes and NAD (P) - dependent enzymes using pyrroloquinoline-enzyme electrodes in a self assembled monolayer configuration: design of new class of amperometric biosensors", Anal. Chem. 66 (9), 1994, pp. 1534- 1539.
[15] Danielsson B. "Enzyme thermistor for food analysis, Wagner. G. Guilbault (Eds)", Food biosensor Analysis, Marcel Dekker, 1994, pp. 173-190.
[16] Appleton A., Gibson T. D., and Woodward J. R., "High temperature stabilization of immobilized glucose oxidase: potential applications in biosensors", Sensors and Actuators, B 43, 1997, pp. 65-69.
[17] Gouda M. D., Kumar M.A., Thakur and Karanth N.G., "Enhancement of Operational stability of an Enzyme Biosensor, for the glucose and sucrose using protein based stabilizing agents", Biosensors and Bioelectronics, 19(6), 2002, pp. 503-507.
[18] Sarath Babu V. R., Kumar M.A., Karanth N.G., and Thakur M. S., "Stabilization of the immobilized glucose oxidase against thermal inactivation by silanization for applications", Biosensors and Bioelectronics, 19 (10), 2004, pp. 132-137.
[19] Sergio Franco. "Design with operational Amplifiers and Analog integrated Circuits", Tata McGraw-Hill, 2002, pp. 60-91.
[20] Muhammad Ali Mazidi and Janice Gillispie Mazidi, "The 8051 Microcontroller and Embedded Systems", Pearson education, 2004, pp. 235-243.
[21] Kilpatric E. S., Ramulay A. G, Smith E. A., " Variations in sample pH and pO2 affect ExacTech meter glucose measurements", Diabet Med, Jun11(5), 1994, pp.506-509.