%0 Journal Article
	%A JiEun Jeong and  Se-Won Kang and  Hee-Ju Hwang and  Sung-Hwa Chae and  Sang-Haeng Choi and  Hong-SeogPark and  YeonSoo Han and  Bok-Reul Lee and  Dae-Hyun Seog and  Yong Seok Lee
	%D 2011
	%J International Journal of Biotechnology and Bioengineering
	%B World Academy of Science, Engineering and Technology
	%I Open Science Index 59, 2011
	%T Construction of cDNALibrary and EST Analysis of Tenebriomolitorlarvae
	%U https://publications.waset.org/pdf/1980
	%V 59
	%X Tofurther advance research on immune-related genes
from T. molitor, we constructed acDNA library and analyzed
expressed sequence taq (EST) sequences from 1,056 clones. After
removing vector sequence and quality checkingthrough thePhred
program (trim_alt 0.05 (P-score>20), 1039 sequences were generated.
The average length of insert was 792 bp. In addition, we identified 162
clusters, 167 contigs and 391 contigs after clustering and assembling
process using a TGICL package. EST sequences were searchedagainst
NCBI nr database by local BLAST (blastx, E
	%P 687 - 689