@article{(Open Science Index):https://publications.waset.org/pdf/1980, title = {Construction of cDNALibrary and EST Analysis of Tenebriomolitorlarvae}, author = {JiEun Jeong and Se-Won Kang and Hee-Ju Hwang and Sung-Hwa Chae and Sang-Haeng Choi and Hong-SeogPark and YeonSoo Han and Bok-Reul Lee and Dae-Hyun Seog and Yong Seok Lee}, country = {}, institution = {}, abstract = {Tofurther advance research on immune-related genes from T. molitor, we constructed acDNA library and analyzed expressed sequence taq (EST) sequences from 1,056 clones. After removing vector sequence and quality checkingthrough thePhred program (trim_alt 0.05 (P-score>20), 1039 sequences were generated. The average length of insert was 792 bp. In addition, we identified 162 clusters, 167 contigs and 391 contigs after clustering and assembling process using a TGICL package. EST sequences were searchedagainst NCBI nr database by local BLAST (blastx, E}, journal = {International Journal of Biotechnology and Bioengineering}, volume = {5}, number = {11}, year = {2011}, pages = {687 - 689}, ee = {https://publications.waset.org/pdf/1980}, url = {https://publications.waset.org/vol/59}, bibsource = {https://publications.waset.org/}, issn = {eISSN: 1307-6892}, publisher = {World Academy of Science, Engineering and Technology}, index = {Open Science Index 59, 2011}, }