Anuar and N. and Markom and M. and Khairedin and S. and Johari and N. A.
Production and Extraction of Quercetin and ()Catechin from Phyllanthus niruri Callus Culture
968 - 971
2012
6
10
International Journal of Biotechnology and Bioengineering
https://publications.waset.org/pdf/16911
https://publications.waset.org/vol/70
World Academy of Science, Engineering and Technology
Quercetin and ()catechin are metabolites present in Phyllanthus niruri plant, have potential in medicinal uses as anticancer and antioxidant agents. Studies on production of quercetin and ()catechin from P. niruri callus culture via in vitro technique were carried out and the results were compared to the intact plant. P. niruri explants were cultured on Murashige and Skoog (MS) solidified media supplemented with several phytohormone combinations for one month. The metabolites were extracted from P. niruri callus and intact plant by using carbon dioxide supercritical fluid extraction (SFE) with ethanol as modifier and solvent extraction techniques. The extracts were analyzed by means of HPLC method. Results showed that P. niruri callus culture was successfully established. The highest content of quercetin (1.72) was found from P. niruri callus grown in media supplemented with 0.8mgL kinetin and 0.2mgL 2,4dicholophenoxyacetic acid (2,4D), which was 1.2 fold higher than intact plant. Meanwhile, the highest amounts of ()catechin (0.63) was found from P. niruri callus grown in media with addition of 0.2mgL 1naphthalene acetic acid (NAA) and 0.8mgL 2,4D. The SFE condition in this study showed better extraction efficiency when higher contents of selected metabolites were found in all SFE extracts compared to the common solvent extracts.
Open Science Index 70, 2012