@article{(Open Science Index):https://publications.waset.org/pdf/16898,
	  title     = {Optimization of Lipase Production Using Bacillus subtilis by Response Surface Methodology},
	  author    = {A. Shyamala Devi and  K. Chitra Devi and  R. Rajendiran},
	  country	= {},
	  institution	= {},
	  abstract     = {A total of 6 isolates of Bacillus subtilis were isolated from oil mill waste collected in Namakkal district, Tamilnadu, India. The isolated bacteria were screened using lipase screening medium containing Tween 80. BS-3 isolate exhibited a greater clear zone than the others, indicating higher lipase activity. Therefore, this isolate was selected for media optimization studies. Ten process variables were screened using Plackett–Burman design and were further optimized by central composite design of response surface methodology for lipase production in submerged fermentation. Maximum lipase production of 16.627 U/min/ml were predicted in medium containing yeast extract (9.3636g), CaCl2 (0.8986g) and incubation periods (1.813 days). A mean value of 16.98 ± 0.2286 U/min/ml of lipase was acquired from real experiments.
},
	    journal   = {International Journal of Biotechnology and Bioengineering},
	  volume    = {6},
	  number    = {9},
	  year      = {2012},
	  pages     = {840 - 845},
	  ee        = {https://publications.waset.org/pdf/16898},
	  url   	= {https://publications.waset.org/vol/69},
	  bibsource = {https://publications.waset.org/},
	  issn  	= {eISSN: 1307-6892},
	  publisher = {World Academy of Science, Engineering and Technology},
	  index 	= {Open Science Index 69, 2012},
	}