Arti Goel and Kanika Sharma
Effect of Euphorbia Pulcherrima Leaf and Inflorescence Extract on Various Cytomorphological Parameters of Aspergillus fumigatus
859 - 862
2013
7
9
International Journal of Biotechnology and Bioengineering
https://publications.waset.org/pdf/16642
https://publications.waset.org/vol/81
World Academy of Science, Engineering and Technology
Microorganisms can be removed, inhibited or killed by physical agents, physical processes or chemical agents but they have their inherent disadvantages such as increased resistance against antibiotics etc. Since, plants have endless ability to synthesize aromatic substances which act as the master agents for plant defense mechanisms against microorganisms, insects and herbivores. Thus, secondary metabolites or phytochemicals obtained from plants can be used as agents of disease control nowadays. In the present study effect of different concentrations of acetone fraction of leaves and alcohol fraction of inflorescence of Euphorbia pulcherrima on various cytomorphological parameters i.e. cell number, mycelium width, conidial size, conidiophore size etc. of Aspergillus fumigatus has been studied. Change in mycelium hyphal cell width, conidium size, conidiophore size etc. was measured with the help of a previously calibrated oculometer. To study effect on morphology, fungal mycelium along with conidiophore and conidia were stained with cotton blue and mounted in lactophenol and observed microscopically. Inhibitory action of the acetone extract of Euphorbia pulcherrima leaf on growth of Aspergillus fumigatus was investigated. Control containing extract free medium supported profuse growth of the fungus. Although decrease in growth was observed even at 3.95μgml but significant inhibition of growth was started at7.81μgml concentration of the extract. Complete inhibition was observed at 15.62μgml and above. Microscopic examination revealed that at 3.95, 7.81 and 15.62μgml extract concentration hyphal cell width was found to be increased from 1.44μm in control to 3.86, 5.24 and 8.98 μm respectively giving a beaded appearance to the mycelium. Vesicle size was reduced from 24.78x20.08μm (control) to 11.34x10.06μm at 3.95μgml concentration. At 7.81 and 15.62μgml concentration no phialides and sterigmata were observed. Inhibitory action of the alcohol extract of inflorescence on the growth of Aspergillus fumigatus was also studied. Control containing extract free medium supported profuse growth of the fungus. Although decrease in growth was observed even at 3.95μgml but complete inhibition was observed at 62.5μgml and above. Microscopic examination revealed that hyphal cell width of Aspergillus fumigatus was found to be increased from 1.67μm in control to 5.84μm at MIC i.e. at 62.5μgml. Vesicle size was reduced from 44.76x 24.22μm (control) to 11.36x 6.80μm at 15.62μgml concentrations. At 31.25 μgml and 62.5μgml concentration no phialides and sterigmata was found. Spore germination was completely found to be inhibited at 3.95μgml concentration. Similarly 92.87 reduction in vesicle size was observed at 15.62μgml concentration. It is evident from the results that plant extracts inhibit fungal growth and this inhibition is concentration dependent.
Open Science Index 81, 2013