Aureobasidium pullulans Used as a Biological Control Agent under Field Conditions Affects the Microbial Quality of Winter Wheat Grain

Authors: Urszula Wachowska, Anna Daria Stasiulewicz-Paluch, Iwona Konopka, Katarzyna Kucharska, Justyna Borowska

Abstract:

The biological activity of A. pullulans isolates against species of the genus Fusarium, bacteria of the genus Azotobacter and pseudomonads colonizing wheat kernels was evaluated. A field experiment was carried out in 2009-2011, in north-eastern Poland. Winter wheat (cv. Bogatka) plants were sprayed with a cell suspension of A. pullulans at a density of 106 - 108 per cm3 water at the stem elongation stage and the heading stage. Untreated plants served as control. The abundance of epiphytic yeasts, bacteria of the genus Azotobacter, pseudomonads and Fusarium pathogens on wheat grain was estimated at harvest and after six months’ storage. The average size of yeast communities was significantly greater on wheat kernels treated with a cell suspension of A. pullulans, compared with control samples. In 2010-2011, biological control reduced the abundance of some species of the genus Fusarium.

Keywords: Aureobasidium pullulans, winter wheat grain, Fusarium, bacteria.

Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1087211

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