@article{(Open Science Index):https://publications.waset.org/pdf/15170,
	  title     = {Production of Milk Clotting Protease by Rhizopus Stolonifer through Optimization of Culture Conditions},
	  author    = {S. Gais and  F. Fazouane and  A. Mechakra},
	  country	= {},
	  institution	= {},
	  abstract     = {The present study describes the biosynthesis of a milkclotting
protease by solid state fermentation (SSF) of a locally
isolated mould, Rhizopus stolonifer. The production medium was
prepared using wheat bran at 50% (w/v). The production conditions
are optimized by varying 7 parameters: carbon and nitrogen sources,
medium moisture, temperature, pH, fermentation time and
inoculum-s size. The maximum enzyme synthesis was measured after
96 h of incubation time at temperature of 28°C. The optimum pH
determined was 6 and the inoculum size was 3.106spores/ml. The
optimum initial moisture content is comprised between 50 to 70%.
The formation of milk clotting protease is enhanced when galactose
and peptone are used at 10% (w/v) and 1% (w/v) concentrations
respectively. The maximum production of milk clotting protease is
120 US/ml.},
	    journal   = {International Journal of Nutrition and Food Engineering},
	  volume    = {3},
	  number    = {6},
	  year      = {2009},
	  pages     = {340 - 344},
	  ee        = {https://publications.waset.org/pdf/15170},
	  url   	= {https://publications.waset.org/vol/30},
	  bibsource = {https://publications.waset.org/},
	  issn  	= {eISSN: 1307-6892},
	  publisher = {World Academy of Science, Engineering and Technology},
	  index 	= {Open Science Index 30, 2009},
	}