@article{(Open Science Index):https://publications.waset.org/pdf/15170, title = {Production of Milk Clotting Protease by Rhizopus Stolonifer through Optimization of Culture Conditions}, author = {S. Gais and F. Fazouane and A. Mechakra}, country = {}, institution = {}, abstract = {The present study describes the biosynthesis of a milkclotting protease by solid state fermentation (SSF) of a locally isolated mould, Rhizopus stolonifer. The production medium was prepared using wheat bran at 50% (w/v). The production conditions are optimized by varying 7 parameters: carbon and nitrogen sources, medium moisture, temperature, pH, fermentation time and inoculum-s size. The maximum enzyme synthesis was measured after 96 h of incubation time at temperature of 28°C. The optimum pH determined was 6 and the inoculum size was 3.106spores/ml. The optimum initial moisture content is comprised between 50 to 70%. The formation of milk clotting protease is enhanced when galactose and peptone are used at 10% (w/v) and 1% (w/v) concentrations respectively. The maximum production of milk clotting protease is 120 US/ml.}, journal = {International Journal of Nutrition and Food Engineering}, volume = {3}, number = {6}, year = {2009}, pages = {340 - 344}, ee = {https://publications.waset.org/pdf/15170}, url = {https://publications.waset.org/vol/30}, bibsource = {https://publications.waset.org/}, issn = {eISSN: 1307-6892}, publisher = {World Academy of Science, Engineering and Technology}, index = {Open Science Index 30, 2009}, }