%0 Journal Article %A Yetti Marlida and Rina Delfita and Neni Gusmanizar and Gita Ciptaan %D 2010 %J International Journal of Bioengineering and Life Sciences %B World Academy of Science, Engineering and Technology %I Open Science Index 41, 2010 %T Identification Characterization and Production of Phytase from Endophytic Fungi %U https://publications.waset.org/pdf/13385 %V 41 %X Phytases are acid phosphatase enzymes, which efficiently cleave phosphate moieties from phytic acid, thereby generating myo-inositol and inorganic phosphate. Thirty four isolates of endophytic fungi to produce of phytases were isolated from leaf, stem and root fragments of soybean. Screening of 34 isolates of endophytic fungi identified the phytases produced by Rhizoctonia sp. and Fusarium verticillioides . The phytase production were the best induced by phytic acid and rice bran compared the others inducer in submerged fermentation medium used. The phytase produced by both Rhizoctonia sp. and F. verticillioides have pH optimum at 4.0 and 5.0 respectively. The characterization of phytase from Fusarium verticillioides showed that temperature optimum was 500C and stability until 600C, the pH optimum 5.0 and pH stability was 2.5 – 6.0, and substrate specificity were rice bran>soybean meal>corn> coconut cake, respectively. %P 360 - 363