TY - JFULL AU - J. Ruenin and S. Sukprasong and R. Phatthanakun and N. Chomnawang and P. Kuntanawat PY - 2012/10/ TI - Fabrication of Microfluidic Device for Quantitative Monitoring of Algal Cell Behavior Using X-ray LIGA Technology T2 - International Journal of Biotechnology and Bioengineering SP - 741 EP - 745 VL - 6 SN - 1307-6892 UR - https://publications.waset.org/pdf/13109 PU - World Academy of Science, Engineering and Technology NX - Open Science Index 69, 2012 N2 - In this paper, a simple microfluidic device for monitoring algal cell behavior is proposed. An array of algal microwells is fabricated by PDMS soft-lithography using X-ray LIGA mold, placed on a glass substrate. Two layers of replicated PDMS and substrate are attached by oxygen plasma bonding, creating a microchannel for the microfluidic system. Algal cell are loaded into the microfluidic device, which provides positive charge on the bottom surface of wells. Algal cells, which are negative charged, can be attracted to the bottom of the wells via electrostatic interaction. By varying the concentration of algal cells in the loading suspension, it is possible to obtain wells with a single cell. Liquid medium for cells monitoring are flown continuously over the wells, providing nutrient and waste exchange between the well and the main flow. This device could lead to the uncovering of the quantitative biology of the algae, which is a key to effective and extensive algal utilizations in the field of biotechnology, food industry and bioenergy research and developments. ER -