%0 Journal Article
	%A Mithilesh Singh and  Rakhi Chaturvedi
	%D 2009
	%J International Journal of Bioengineering and Life Sciences
	%B World Academy of Science, Engineering and Technology
	%I Open Science Index 27, 2009
	%T An Efficient Protocol for Cyclic Somatic Embryogenesis in Neem (Azadirachta indica A Juss.)
	%U https://publications.waset.org/pdf/12584
	%V 27
	%X Neem is a highly heterozygous and commercially
important perennial plant. Conventionally, it is propagated by seeds
which loose viability within two weeks. Strictly cross pollinating
nature of the plant causes serious barrier to the genetic improvement
by conventional methods. Alternative methods of tree improvement
such as somatic hybridization, mutagenesis and genetic
transformation require an efficient in vitro plant regeneration system.
In this regard, somatic embryogenesis particularly secondary somatic
embryogenesis may offer an effective system for large scale plant
propagation without affecting the clonal fidelity of the regenerants. It
can be used for synthetic seed production, which further bolsters
conservation of this tree species which is otherwise very difficult
The present report describes the culture conditions necessary to
induce and maintain repetitive somatic embryogenesis, for the first
time, in neem. Out of various treatments tested, the somatic embryos
were induced directly from immature zygotic embryos of neem on
MS + TDZ (0.1 μM) + ABA (4 μM), in more than 76 % cultures.
Direct secondary somatic embryogenesis occurred from primary
somatic embryos on MS + IAA (5 μM) + GA3 (5 μM) in 12.5 %
cultures. Embryogenic competence of the explant as well as of the
primary embryos was maintained for a long period by repeated
subcultures at frequent intervals. A maximum of 10 % of these
somatic embryos were converted into plantlets.
	%P 133 - 135