TY - JFULL AU - N. B. Dhayanithi and T. T. Ajith Kumar and T. Balasubramanian PY - 2012/9/ TI - In Vitro and Experimental Screening of Mangrove Herbal Extract against Vibrio Alginolyticus in Marine Ornamental Fish T2 - International Journal of Marine and Environmental Sciences SP - 597 EP - 602 VL - 6 SN - 1307-6892 UR - https://publications.waset.org/pdf/12422 PU - World Academy of Science, Engineering and Technology NX - Open Science Index 68, 2012 N2 - Present study summarizes the control of Vibrio alginolyticus infection in hatchery reared Clownfish, Amphiprion sebae with the extract of the mangrove plant, Avicennia marina. Fishes with visible symptoms of hemorrhagic spots were chosen and the genomic DNA of the causative bacterium was isolated and sequenced based on 16S rDNA gene. The in vitro assay revealed that a fraction of A. marina leaf extract elucidated with ethyl acetate: methanol (6:4) showed a high activity (28 mm) at 125 μg/ml concentrations. About 4 % of the fraction fed along with live V. alginolyticus was significantly decreased the cumulative mortality (P ER -