%0 Journal Article
	%A Darren C-W. Tan and  Partha Roy
	%D 2009
	%J International Journal of Bioengineering and Life Sciences
	%B World Academy of Science, Engineering and Technology
	%I Open Science Index 29, 2009
	%T Glucose-dependent Functional Heterogeneity In β-TC-6 Murine Insulinoma
	%U https://publications.waset.org/pdf/11082
	%V 29
	%X To determine if the murine insulinoma, β-TC-6, is a
suitable substitute for primary pancreatic β-cells in the study of β-
cell functional heterogeneity, we used three distinct functional assays
to ascertain the cell line-s response to glucose or a glucose analog.
These assays include: (i) a 2-NBDG uptake assay; (ii) a calcium
influx assay, and; (iii) a quinacrine secretion assay. We show that a
population of β-TC-6 cells endocytoses the glucose analog, 2-
NBDG, at different rates, has non-uniform intracellular calcium ion
concentrations and releases quinacrine at different rates when
challenged with glucose. We also measured the Km for β-TC-6
glucose uptake to be 46.9 mM and the Vm to be 8.36 x 10-5
mmole/million cells/min. These data suggest that β-TC-6 might be
used as an alternative to primary pancreatic β-cells for the study of
glucose-dependent β-cell functional heterogeneity.
	%P 254 - 257