@article{(Open Science Index):https://publications.waset.org/pdf/11082,
	  title     = {Glucose-dependent Functional Heterogeneity In β-TC-6 Murine Insulinoma},
	  author    = {Darren C-W. Tan and  Partha Roy},
	  country	= {},
	  institution	= {},
	  abstract     = {To determine if the murine insulinoma, β-TC-6, is a
suitable substitute for primary pancreatic β-cells in the study of β-
cell functional heterogeneity, we used three distinct functional assays
to ascertain the cell line-s response to glucose or a glucose analog.
These assays include: (i) a 2-NBDG uptake assay; (ii) a calcium
influx assay, and; (iii) a quinacrine secretion assay. We show that a
population of β-TC-6 cells endocytoses the glucose analog, 2-
NBDG, at different rates, has non-uniform intracellular calcium ion
concentrations and releases quinacrine at different rates when
challenged with glucose. We also measured the Km for β-TC-6
glucose uptake to be 46.9 mM and the Vm to be 8.36 x 10-5
mmole/million cells/min. These data suggest that β-TC-6 might be
used as an alternative to primary pancreatic β-cells for the study of
glucose-dependent β-cell functional heterogeneity.},
	    journal   = {International Journal of Bioengineering and Life Sciences},
	  volume    = {3},
	  number    = {5},
	  year      = {2009},
	  pages     = {254 - 257},
	  ee        = {https://publications.waset.org/pdf/11082},
	  url   	= {https://publications.waset.org/vol/29},
	  bibsource = {https://publications.waset.org/},
	  issn  	= {eISSN: 1307-6892},
	  publisher = {World Academy of Science, Engineering and Technology},
	  index 	= {Open Science Index 29, 2009},
	}