WASET
	%0 Journal Article
	%A Congmin Z. Xie and  Terry Healy and  Peter Robinson and  Kevin Stewart
	%D 2008
	%J International Journal of Environmental and Ecological Engineering
	%B World Academy of Science, Engineering and Technology
	%I Open Science Index 20, 2008
	%T Determination of EDTA in Dairy Wastewater and Adjacent Surface Water
	%U https://publications.waset.org/pdf/10699
	%V 20
	%X An HPLC-UV analytical method was developed to
determine ethylenediaminetetraacetic acid (EDTA) in dairy
wastewater and surface water. The optimizing separation was achieved
by reversed–phase ion-pair liquid chromatography on a C18 column
using methanol as mobile phase solvent, tetrabutylammonium bromide
as the ion-pair reagent in pH 3.3 formate buffer solution at a flow rate
of 0.9 mL min-1 with a UV detector at 265 nm. No interference of Ca,
Mg or NO3
- was detected. Method performance was evaluated in terms
of linearity, repeatability and reproducibility. The method detection
limit was 5 μg L-1. The contents of EDTA in dairy effluents were 72 ~
261 μg L-1 at a large dairy site. A change of EDTA concentration was
observed downstream of the dairy effluent discharge, but this was well
under the predicted no effect concentration for aquatic ecosystem.
	%P 82 - 86