TY - JFULL AU - Jing Zhang and Lian Wu and Jie-ming Qu and Chun-xue Bai and Mervyn J Merrilees and Peter N Black PY - 2011/8/ TI - Pro-inflammatory Phenotype of COPD Fibroblasts not Compatible with Repair in COPD Lung T2 - International Journal of Medical and Health Sciences SP - 262 EP - 274 VL - 5 SN - 1307-6892 UR - https://publications.waset.org/pdf/10175 PU - World Academy of Science, Engineering and Technology NX - Open Science Index 55, 2011 N2 - COPD is characterized by loss of elastic fibers from small airways and alveolar walls, with the decrease in elastin increasing with disease severity. It is unclear why there is a lack of repair of elastic fibers. We have examined fibroblasts cultured from lung tissue from normal and COPD subjects to determine if the secretory profile explains lack of tissue repair. In this study, fibroblasts were cultured from lung parenchyma of bronchial carcinoma patients with varying degrees of COPD; controls (non-COPD, n=5), mild COPD (GOLD 1, n=5) and moderate-severe COPD (GOLD 2-3, n=12). Measurements were made of proliferation, senescence-associated beta-galactosidase-1, mRNA expression of IL-6, IL-8, MMP-1, tropoelastin and versican, and protein levels for IL-6, IL-8, PGE2, tropoelastin, insoluble elastin, and versican. It was found that GOLD 2-3 fibroblasts proliferated more slowly (p ER -