Tiancheng Lan
Construction of a Fusion Gene Carrying E10A and K5 with 2A PeptideLinked by Using Overlap Extension PCR
96 - 100
2021
15
1
International Journal of Biomedical and Biological Engineering
https://publications.waset.org/pdf/10011780
https://publications.waset.org/vol/169
World Academy of Science, Engineering and Technology
E10A is a kind of replicationdefective adenovirus which carries the human endostatin gene to inhibit the growth of tumors. Kringle 5(K5) has almost the same function as angiostatin to also inhibit the growth of tumors since they are all the byproduct of the proteolytic cleavage of plasminogen. Tumor size increasing can be suppressed because both of the endostatin and K5 can restrain the angiogenesis process. Therefore, in order to improve the treatment effect on tumor, 2A peptide is used to construct a fusion gene carrying both E10A and K5. Using 2A peptide is an ideal strategy when a fusion gene is expressed because it can avoid many problems during the expression of more than one kind of protein. The overlap extension PCR is also used to connect 2A peptide with E10A and K5. The final construction of fusion gene E10A2AK5 can provide a possible new method of the antiangiogenesis treatment with a better expression performance.
Open Science Index 169, 2021