The Inhibition of Relapse of Orthodontic Tooth Movement by NaF Administration in Expressions of TGF-β1, Runx2, Alkaline Phosphatase and Microscopic Appearance of Woven Bone
The prevalence of post-treatment relapse in orthodontics in the community is high enough; therefore, relapses in orthodontic treatment must be prevented well. The aim of this study is to experimentally test the inhibition of relapse of orthodontics tooth movement in NaF of expression TGF-β1, Runx2, alkaline phosphatase (ALP) and microscopic of woven bone. The research method used was experimental laboratory research involving 30 rats, which were divided into three groups. Group A: rats were not given orthodontic tooth movement and without NaF. Group B: rats were given orthodontic tooth movement and without 11.5 ppm by topical application. Group C: rats were given orthodontic tooth movement and 11.75 ppm by topical application. Orthodontic tooth movement was conducted by applying ligature wires of 0.02 mm in diameter on the molar-1 (M-1) of left permanent maxilla and left insisivus of maxilla. Immunohistochemical examination was conducted to calculate the number of osteoblast to determine TGF β1, Runx2, ALP and haematoxylin to determine woven bone on day 7 and day 14. Results: It was shown that administrations of Natrium Fluoride topical application proved effective to increase the expression of TGF-β1, Runx2, ALP and to increase woven bone in the tension area greater than administration without natrium fluoride topical application (p < 0.05), except the expression of ALP on day 7 and day 14 which was significant. The results of the study show that NaF significantly increases the expressions of TGF-β1, Runx2, ALP and woven bone. The expression of the variables enhanced on day 7 compared on that on day 14, except ALP. Thus, it can be said that the acceleration of woven bone occurs on day 7.
Digital Object Identifier (DOI): doi.org/10.5281/zenodo.1314562Procedia APA BibTeX Chicago EndNote Harvard JSON MLA RIS XML ISO 690 PDF Downloads 605
 Proffit WR. 2007. The Biologic Basis of Orthodontic Treatment in Contemporary Orthodontics. 4 th ed. Canada. Elsevier. pp 331-41.
 Nanda R and Kapila S. 2010. Current Therapy in Orthodontics.1 st ed. St. Louis. Missouri. pp 326-27.
 Indayani DE 2002. Benefits acid Omega 3 fatty polysaturated post care orthodontics. Magazine Scientific Medical Teeth. Edition Special Foril; 357-61.
 Sakallioglu EE, Muglali M., Bas B., Gulbahar MY, 2014. Effecs of excessive fluoride on bone turnover in the mandible: An immunohistochemical Study in Rabbit s. Research Report Fluoride 47 (1): 23-30.
 Kebsch M, Wilkinson M, Petocz P. 2005. Am J Orthod and dentofacial Orthop. 131: 515-524.
 FQ Jing, Wang Q, Liu TL, Guo LY, Liu H.2006. Effects of fluoride overdosed on rat 's insicor expression of matrix metalloproteinase-20 and tissue inhibitors of metalloproteinase-2. Hua XI Kou Qiang Yi Xue Za Zhi 243: 199-20.
 Henneman S, Von den Hoff JW, JC 2008. Maltha Mechanobiology of tooth movement. Eur J Orthod. 30: 299-306.
 Rich FA., Hamamci N., G. Basaran, Dogree M., Yildirim TT.2010. TNF-α, IL-1 β and IL-1 8 levels in early leveling tooth movement Orthodontic Treatment. Journal of International Dental and Medical Research. 3 (3): 116-21.
 Krisnan V, Davidovitch Z. 2009. Celluler, moleculer ang tissue-level reactions to orthodontic force. Am J Orthod dentofacial Orthop 129: 469e1-4 69e32.
 BN Nayak 2013. Molecular Biology of Orthodontic Tooth Movement. Journal of Dentistry and Oral Health. 1: 1-12.
 Andrade I, Taddei S, Souza P. 2012. Inflammation and Tooth Movement: The Role of Cytokines, chemokines, and Growth Factors. 18: 257-269.
 Lerner UH. 2012. Osteobalsts, osteoclasts, and Osteocytes: Their Intimate Unvelling-Associated Responses to Applied Orthodontic Forces 18: 237- 248.
 Kwon TG, Zao X, Yang Q, Go C, Zhao G, Francechi RT, 2011. NIH Public Access .12: 3582-3593.
 H. Huang, Williams RC, and Kyrkanides S. 2014. Accelerated orthodontic tooth movement: Molecular mechanisms. American Journal of Orthodontics and dentofacial Orthopedics .146: 620-32.
 L Walsh J. 2006. Home care self-applied fluoride products: current concepts for maximal effetiveness. Dental Practice: 66-67.
 Herschel S, 2007. The need for toothpaste with fluoride Lower than conventional consentrations for Preschool-aged Chidren, Journal of Public Health Dentistry, 52 (4): 216-221.
 Nicholas BL and Christopher M. 2015. Functional Monomerization of a CIC-Type Fluoride Trnasporter. J Mol Biol 427 (22): 3607-3612.
 Worrengton A, Roozegar S. 2004. Regenerative Periodontal therapics, Review, Department of Periodontology, Institute of Odontology. Karokischan Institute Stockholm.
 Krisnan V, Davidovitch Z. 2015. Biological Mechanisms of Tooth Movement. 2 nd. John Wiley. & Sons Ltd.USA.
 Chang YC, Yang SF, CC Lai. 2002. Regulatons of Matrix Mettalopoteinase production by citokynes, pharmalogycal agents and periodontalpathogens in human periodontal ligament fibroblasts in culture. Journal of Periodontal Research 37, 196-203.
 Dudic A, Kiliaridis S, Morbelli A, and Glannopoulou C.2006. Comparison between tension and compression sides Europen Journal of Oral Sciences .114.416-422.
 Stein GS, Lian JB, van Wijnen AJ, Stein JL, M. Montecino, Jawed A. Zaidi SK, DW Young, Choi JY, 2004. RUNX Pockwinse SM-2 control of organization, assembly and activity of the regulatory machinery for skeletal gene expression. Oncogene, 23: 4315-29
 T. Fujita, Y. Azuma, R. 2004. Fukuyama RUNX-2 induces osteoblast and chondrocyte differentiation and enhances Reviews their migration by coupling with P13K-Akt signaling. Biol J. Cell. 166: 85-95
 Dogaan A A, Bolpaca P.2009. Evaluation of Craniofacial morphology oh children with dental fluorosis in early dentition period. Eur J Dent .3; 304-13.
 Robling AG, Castillo A B, C Tunner H.2006. Biomechanical and molecular regulation of bone remodeling. Ann Biomed Eng .8; 455-98.
 Karsenty G. 2003. The Complexities of skeletal biology, Nature. 433316-18
 Kuru L, Griffiths GS, Petrie A, Olsen I, 1999. Alkaline Phosphatase acti is upregulated in regenerating human cells periodontal .34; 123-7
 Coleman JE, 1992. Structure and mechanism of Alkaline Phosphatase. Annu Rev Biophys Biomol Struet. 21; 444-83.
 Everelt E T. 2011. Fluorides effect on the formation of teeth and Bones, and the influence of Genetics. J Dent Res. 90 (5); 552-60.
 D 'Apuzo F., Cappablanca S., Clavarella D., Monsurro A., Biatti US and Perillo. 2013. Biomarkers of periodontal Tisuue Remodelling during Orthodontic Tooth Movement in Mice and Men: Overview and Clinical Relevance. The Scientific World Journal.
 Monjo M, Lamolle SF, Lyngtadaas SP, Ronold HJ, J Ellingsen E. 2008. In vivo expression of osteogenic markers and bone density material at the surface of fluoride- modified titanium implants. Biomaterials. 29: 3771-80.
 Shebani A., Valaci N., Vasooghi M., Noorbakhsh M., 2010. Incidence of relaps in Orthodontic Treatments and Related factors. Journal of Research in Dental Sciences.7(2): 32-41.
 Tanya, J Franzen., Sherif, E Zahra., Abbadi, El-Kadi., and Vaska, Vandevska-Radunovic. 2014. The Influence of Low-level Laser on Orthodontic Relapse in Rats. European Journal of Orthodontics. vol 37(1): 111-117.