Marek Halenár and Eva Tvrdá and Simona Baldovská and Ľubomír Ondruška and Peter Massányi and Adriana Kolesárová
In vitro Effects of Viscum album on the Functionality of Rabbit Spermatozoa
108 - 112
2017
11
2
International Journal of Animal and Veterinary Sciences
https://publications.waset.org/pdf/10006265
https://publications.waset.org/vol/122
World Academy of Science, Engineering and Technology
This study aimed to assess the in vitro effects of different concentrations of the Viscum album extract on the motility, viability, and reactive oxygen species (ROS) production by rabbit spermatozoa during different time periods (0, 2, and 8h). Spermatozoa motility was assessed by using the CASA (Computer aided sperm analysis) system. Cell viability was evaluated by using the metabolic activity MTT assay, and the luminolbased luminometry was applied to quantify the ROS formation. The CASA analysis revealed that low Viscum concentrations were able to prevent a rapid decline of spermatozoa motility, especially in the case of concentrations ranging between 1 and 5 µgmL (P<0.05 with respect to time 8h). At the same time, concentrations ranging between 1 and 100 µgmL of the extract led to a significant preservation of the cell viability (P<0.05 in case of 5, 50 and 100 µgmL; P<0.01 with respect to 1 and 10 µgmL, time 8h). 1 and 5 µgmL of the extract exhibited antioxidant characteristics, translated into a significant reduction of the ROS production, particularly notable at time 8h (P<0.01). The results indicate that the Viscum extract is capable of delaying the damage inflicted to the spermatozoon by the in vitro environment.
Open Science Index 122, 2017