@article{(Open Science Index):https://publications.waset.org/pdf/10002330,
	  title     = {Removal of Aggregates of Monoclonal Antibodies by Ion Exchange Chromatography},
	  author    = {Ishan Arora and  Anurag S. Rathore},
	  country	= {},
	  institution	= {},
	  abstract     = {The primary objective of this work was to study the
effect of resin chemistry, pH and molarity of binding and elution
buffer on aggregate removal using Cation Exchange Chromatography
and find the optimum conditions which can give efficient aggregate
removal with minimum loss of yield. Four different resins were used
for carrying out the experiments: Fractogel EMD SO3
-(S), Fractogel EMD COO-(M), Capto SP ImpRes and S Ceramic HyperD. Runs
were carried out on the AKTA Avant system. Design of Experiments
(DOE) was used for analysis using the JMP software. The
dependence of the yield obtained using different resins on the
operating conditions was studied. Success has been achieved in
obtaining yield greater than 90% using Capto SP ImpRes and
Fractogel EMD COO-(M) resins. It has also been found that a change
in the operating conditions generally has different effects on the
yields obtained using different resins.
},
	    journal   = {International Journal of Chemical and Molecular Engineering},
	  volume    = {8},
	  number    = {3},
	  year      = {2014},
	  pages     = {257 - 261},
	  ee        = {https://publications.waset.org/pdf/10002330},
	  url   	= {https://publications.waset.org/vol/87},
	  bibsource = {https://publications.waset.org/},
	  issn  	= {eISSN: 1307-6892},
	  publisher = {World Academy of Science, Engineering and Technology},
	  index 	= {Open Science Index 87, 2014},
	}